Evidence map›Paper›PMID 42415068›Full record

ArticleJournal of translational medicine2026

LncRNA AP000654.1 attenuates cerebral ischemia-reperfusion injury by regulating mitophagy-mediated NLRP3 inflammasome activation.

Chi Zhang, Yixiang Cui, Zhaojing Zhang, Manyue Zhang, Qiong Zhang, Yanyang Huang, Yatian Xu, Tianyi Han, Dongzhi Yang, Ying He

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Article in Journal of translational medicine, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

10 authors.

Chi Zhang *Department of Medical Genetics and Cell Biology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, China.
Yixiang Cui *Department of Medical Genetics and Cell Biology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, China.
Zhaojing ZhangDepartment of Medical Genetics and Cell Biology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, China.
Manyue ZhangDepartment of Medical Genetics and Cell Biology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, China.
Qiong ZhangDepartment of Medical Genetics and Cell Biology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, China.
Yanyang HuangDepartment of Medical Genetics and Cell Biology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, China.
Yatian XuDepartment of Medical Genetics and Cell Biology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, China.
Tianyi HanDepartment of Medical Genetics and Cell Biology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, China.
Dongzhi YangSchool of Life Sciences, Zhengzhou University, Zhengzhou, 450001, China. ydongzhi@zzu.edu.cn.
Ying HeDepartment of Medical Genetics and Cell Biology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, China. heying39@zzu.edu.cn.

Funding

National Natural Science Foundation of China 82201478National Natural Science Foundation of China U2004114
6 · The paper itself

Abstract

backgroundIschemic stroke (IS) remains a leading cause of disability worldwide, and its therapeutic window is very narrow. Ischemia-reperfusion injury exacerbates secondary brain damage. Microglial NLRP3 inflammasome activation drives neuroinflammation, yet its upstream regulation requires further elucidation. We previously identified lncRNA AP000654.1 as significantly downregulated in the peripheral blood of stroke patients, prompting our investigation into its potential role in microglial biology.

methodsUsing an in vitro oxygen-glucose deprivation/reperfusion (OGD/R) model in HMC3 human microglial cells, we investigated the role of lncRNA AP000654.1-previously identified as significantly downregulated in peripheral blood of stroke patients-in regulating microglial inflammatory responses.

resultsOGD/R treatment upregulated NLRP3 inflammasome-related genes, induced pyroptosis-associated changes, and altered mitophagy markers. LncRNA AP000654 0.1 overexpression suppressed this excessive mitophagy by inhibiting the PINK1-Parkin pathway, thereby improving mitochondrial function. This suppression subsequently attenuated NLRP3 inflammasome assembly and pyroptosis. Crucially, enforcing mitophagy with rapamycin exacerbated the injury, which was effectively rescued by lncRNA AP000654.

conclusionLncRNA AP000654.1 is associated with suppression of excessive mitophagy-assocated mitochondrial stress and NLRP3 inflammasome activation in microglia, suggesting its potential relevance as a therapeutic target in ischemic stroke.

Indexed as

Brain IschemiaInflammasomesMitophagyNLR Family, Pyrin Domain-Containing 3 ProteinReperfusion InjuryRNA, Long NoncodingCell LineGlucoseHumansMicrogliaMitochondriaOxygenProtein KinasesPTEN-Induced Putative KinasePyroptosisSignal TransductionGlucoseInflammasomesNLR Family, Pyrin Domain-Containing 3 ProteinNLRP3 protein, humanOxygenparkin proteinProtein KinasesPTEN-Induced Putative KinaseRNA, Long NoncodingUbiquitin-Protein LigasesIschemic strokelncRNA AP000654.1MicrogliaMitophagyNeuroinflammationNLRP3 inflammasome

Identifiers

PMID42415068
PMCPMC13628737

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