Enterococcus faecalis NBRC 100481 Protects the Intestinal Barrier via α-catenin/HMP-1 in Caenorhabditis elegans.

Ruina Xu, Jingrui Luo, Chang Huang, Suxiang Li, Chunyan Wu, Qiao Qin, Yong Cao, Yunjiao Chen

Abstract read

In one paragraph

Article in Probiotics and antimicrobial proteins, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

8 authors.

Ruina XuGuangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, College of Food Science, South China Agricultural University, Guangzhou, Guangdong, 510640, China.

Jingrui LuoGuangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, College of Food Science, South China Agricultural University, Guangzhou, Guangdong, 510640, China.

Chang HuangGuangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, College of Food Science, South China Agricultural University, Guangzhou, Guangdong, 510640, China.

Suxiang LiGuangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, College of Food Science, South China Agricultural University, Guangzhou, Guangdong, 510640, China.

Chunyan WuGuangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, College of Food Science, South China Agricultural University, Guangzhou, Guangdong, 510640, China.

Qiao QinGuangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, College of Food Science, South China Agricultural University, Guangzhou, Guangdong, 510640, China.

Yong CaoGuangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, College of Food Science, South China Agricultural University, Guangzhou, Guangdong, 510640, China.

Yunjiao ChenGuangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, College of Food Science, South China Agricultural University, Guangzhou, Guangdong, 510640, China. yunjiaochen@scau.edu.cn.ORCID https://orcid.org/0000-0001-5674-6547

Funding

General project of the Natural Science Foundation of Guangdong Province, China 2026A1515012433

6 · The paper itself

Abstract

Intestinal barrier disruption is a hallmark of gastrointestinal disorders and is also linked to metabolic diseases, posing serious health risks. Probiotic-based interventions have thus attracted growing attention. This study isolated Enterococcus faecalis NBRC 100481 from recovered colitis mice after dietary intervention with pentagalloyl glucose (PGG), and evaluated its protective effects and PGG's regulatory role. Fermentation revealed that PGG enhanced metabolic activity of E. faecalis NBRC 100481 and promoted production of bioactive metabolites such as N-acetylglutamic acid and 4-hydroxybenzaldehyde, which reduced intestinal permeability in Caenorhabditis elegans model. The strain also modulated host responses by preserving epithelial structure and suppressing aberrant expression of VHA-6. Its protective effects were abolished in zoo-1 and hmp-1 mutant worms, suggesting a dependence on host adhesion protein-related pathways. Notably, it attenuated the injury-induced upregulation of α-catenin/HMP-1 in worms with intestinal injury. Collectively, E. faecalis NBRC 100481, modulated by PGG, enhances intestinal barrier function through coordinated microbial metabolite production and host regulatory pathways, with the α-catenin/HMP-1 axis involved as a downstream component. This study offers new insights into polyphenol-probiotic-host interactions and provides strain-level evidence for probiotic-based barrier repair strategies.

Indexed as

Enterococcus faecalis NBRC 100481Intestinal barrierPentagalloyl glucoseα-catenin/HMP-1

Identifiers

PMID42412325

DOI10.1016/j.fbio.2024.103688

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