Evidence map›Paper›PMID 42411381›Full record

ArticleGut microbes2026

Isolation of Bacteria and Fungi from Human Pancreatic Tumors and Duodenum.

Dominik Awad, Holly Attebury, Ryan Hong, Kwi Kim, Li Zhang, Allison Bischoff, Sajan Achi, Aaron denDekker, Nick Lesniak, Stephanie The and 16 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Gut microbes, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

26 authors.

Dominik AwadDepartment of Molecular & Integrative Physiology, University of Michigan, Ann Arbor, MI, USA.ORCID 0000-0002-7386-2061
Holly AtteburyThe Rogel and Blondy Center for Pancreatic Cancer at the University of Michigan Health Rogel Cancer Center, Ann Arbor, MI, USA.
Ryan HongDepartment of Molecular & Integrative Physiology, University of Michigan, Ann Arbor, MI, USA.
Kwi KimDepartment of Internal Medicine, Division of Infectious Diseases, University of Michigan, Ann Arbor, MI, USA.
Li ZhangDepartment of Molecular & Integrative Physiology, University of Michigan, Ann Arbor, MI, USA.
Allison BischoffGraduate Program in Cancer Biology, University of Michigan, Ann Arbor, MI, USA.
Sajan AchiThe Rogel and Blondy Center for Pancreatic Cancer at the University of Michigan Health Rogel Cancer Center, Ann Arbor, MI, USA.
Aaron denDekkerDepartment of Molecular & Integrative Physiology, University of Michigan, Ann Arbor, MI, USA.
Nick LesniakDepartment of Biostatistics, University of Michigan School of Public Health, Ann Arbor, MI, USA.
Stephanie TheDepartment of Biostatistics, University of Michigan School of Public Health, Ann Arbor, MI, USA.
Joseph A Nieto CarrionDepartment of Molecular & Integrative Physiology, University of Michigan, Ann Arbor, MI, USA.
Noah S NelsonDepartment of Molecular & Integrative Physiology, University of Michigan, Ann Arbor, MI, USA.
Christopher StrayhornThe Rogel and Blondy Center for Pancreatic Cancer at the University of Michigan Health Rogel Cancer Center, Ann Arbor, MI, USA.
Brian D GriffithThe Rogel and Blondy Center for Pancreatic Cancer at the University of Michigan Health Rogel Cancer Center, Ann Arbor, MI, USA.
Hannah R WatkoskeThe Rogel and Blondy Center for Pancreatic Cancer at the University of Michigan Health Rogel Cancer Center, Ann Arbor, MI, USA.
Carlos E EspinozaThe Rogel and Blondy Center for Pancreatic Cancer at the University of Michigan Health Rogel Cancer Center, Ann Arbor, MI, USA.
Nicole PetersonDepartment of Internal Medicine, Division of Hematology-Oncology, University of Michigan, Ann Arbor, MI, USA.
Miranda LenardDepartment of Internal Medicine, Division of Hematology-Oncology, University of Michigan, Ann Arbor, MI, USA.
Alexander MuirBen May Department for Cancer Research, University of Chicago, Chicago, IL, USA.
Vaibhav SahaiDepartment of Internal Medicine, Division of Hematology-Oncology, University of Michigan, Ann Arbor, MI, USA.
Gen LiDepartment of Biostatistics, University of Michigan School of Public Health, Ann Arbor, MI, USA.
Timothy L FrankelThe Rogel and Blondy Center for Pancreatic Cancer at the University of Michigan Health Rogel Cancer Center, Ann Arbor, MI, USA.
Marina Pasca di MaglianoThe Rogel and Blondy Center for Pancreatic Cancer at the University of Michigan Health Rogel Cancer Center, Ann Arbor, MI, USA.
Costas A LyssiotisDepartment of Molecular & Integrative Physiology, University of Michigan, Ann Arbor, MI, USA.
Thomas M SchmidtDepartment of Internal Medicine, Division of Infectious Diseases, University of Michigan, Ann Arbor, MI, USA.
Donnele DaleyThe Rogel and Blondy Center for Pancreatic Cancer at the University of Michigan Health Rogel Cancer Center, Ann Arbor, MI, USA.

Funding

XenograftP30CA046592 · NCI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Eric R. Fearon · 1988 to 2026
$178.2M
RESEARCH TRAINING IN EXPERIMENTAL IMMUNOPATHOLOGYT32AI007413 · NIAID · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Bethany B. Moore · 1993 to 2026
$9.8M
Training Program in Tumor MicroenvironmentT32CA009676 · NCI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Maria G Castro, Analisa Virginia DiFeo · 1992 to 2026
$7.3M
University of Michigan Surgical Oncology Research Training ProgramT32CA009672 · NCI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI JACQUELINE SARA JERUSS, WEIPING ZOU · 1991 to 2026
$6.0M
Training in Basic and Translational Digestive SciencesT32DK094775 · NIDDK · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI JOHN Y KAO, LINDA C. SAMUELSON · 2012 to 2026
$3.6M
Intratumoral Metabolic Crosstalk Promotes Therapeutic Resistance in Pancreatic CancerR37CA237421 · NCI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Costas Andreas Lyssiotis · 2020 to 2026
$2.6M
CTSA Predoctoral T32 at the University of MichiganT32TR004371 · NCATS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI VICKI L ELLINGROD, MICHAEL Allan HOLINSTAT · 2023 to 2026
$2.2M
Stromal metabolism promotes therapeutic resistance in pancreatic cancerR01CA248160 · NCI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI LYSSIOTIS, COSTAS ANDREAS · 2020 to 2024
$2.0M
Targeting metabolic stress to induce pancreatic tumor cell deathR01CA244931 · NCI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI LYSSIOTIS, COSTAS ANDREAS · 2020 to 2024
$1.9M
Microbial Dysbiosis in Pancreatic Cancer Initiation and ProgressionK08CA282374 · NCI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Donnele Daley · 2023 to 2026
$1.1M
The role of mesothelial-derived cancer-associated fibroblasts in the pathogenesis of pancreatic ductal adenocarcinomaF31CA284505 · NCI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Allison Bischoff · 2024 to 2026
$144k
The role of bacterial dysbiosis and its influence on pancreatic cancer cell behaviorF31CA306283 · NCI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI ATTEBURY, HOLLY · 2025 to 2025
$44k
NCATS NIH HHS T32 TR004371NCI NIH HHS F31 CA284505NCI NIH HHS F31 CA306283NCI NIH HHS K08 CA282374NCI NIH HHS P30 CA046592NCI NIH HHS R01 CA244931NCI NIH HHS R01 CA248160NCI NIH HHS R37 CA237421NCI NIH HHS T32 CA009672NCI NIH HHS T32 CA009676NIAID NIH HHS T32 AI007413NIDDK NIH HHS T32 DK094775
6 · The paper itself

Abstract

Pancreatic ductal adenocarcinoma has a unique tumor microbiome, and the depletion of gut bacteria or fungi using antibiotic/antifungal cocktails has been shown to decrease pancreatic tumor burden in mice. However, functional studies evaluating the role of tumor-associated microbes are few due to the limited availability of clinically relevant microbiota. Here, we describe in detail an effective workflow for the isolation of bacteria and fungi from the duodenum and tumor of pancreatic cancer patients, specifically optimized for cryopreserved, low biomass samples. Using this workflow we also isolated microbiota from normal pancreatic tissue and duodenum from organ donors, and we confirmed the presence of bacteria and fungi isolated from tissue samples with 16S and ITS sequencing analysis. Isolation and sequencing results show distinct similarities between the pancreatic and duodenal microbiomes and highlight unique bacterial strains that survive in the tumor microenvironment. As a proof of concept, we characterized a select

Indexed as

BacteriaCarcinoma, Pancreatic DuctalDuodenumFungiPancreatic NeoplasmsGastrointestinal MicrobiomeHumansPancreasRNA, Ribosomal, 16SRNA, Ribosomal, 16SKlebsiellametabolomicsmicrobial metabolitesmicrobiomePancreatic cancerPDAC

Identifiers

PMID42411381
PMCPMC13348923

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.