Evidence map›Paper›PMID 42410340›Full record

ArticleBMC plant biology2026

Synergistic effects of melatonin and 24-epibrassinolide on salt stress tolerance in Medicago sativa (Alfalfa) seedlings.

Ruojun Sun, Xinyu Hou, Fei Han, Hui Wang, Hong Pan, Quangang Yang, Zhongchen Yang, Yanhong Lou, Yuping Zhuge

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC plant biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Ruojun SunNational Engineering Research Center for Efficient Utilization of Soil and Fertilizer Resources, College of Resources and Environment, Shandong Agricultural University, Tai' an, 271018, China.
Xinyu HouNational Engineering Research Center for Efficient Utilization of Soil and Fertilizer Resources, College of Resources and Environment, Shandong Agricultural University, Tai' an, 271018, China.
Fei HanNational Engineering Research Center for Efficient Utilization of Soil and Fertilizer Resources, College of Resources and Environment, Shandong Agricultural University, Tai' an, 271018, China.
Hui WangNational Engineering Research Center for Efficient Utilization of Soil and Fertilizer Resources, College of Resources and Environment, Shandong Agricultural University, Tai' an, 271018, China.
Hong PanNational Engineering Research Center for Efficient Utilization of Soil and Fertilizer Resources, College of Resources and Environment, Shandong Agricultural University, Tai' an, 271018, China.
Quangang YangNational Engineering Research Center for Efficient Utilization of Soil and Fertilizer Resources, College of Resources and Environment, Shandong Agricultural University, Tai' an, 271018, China.
Zhongchen YangNational Engineering Research Center for Efficient Utilization of Soil and Fertilizer Resources, College of Resources and Environment, Shandong Agricultural University, Tai' an, 271018, China.
Yanhong LouNational Engineering Research Center for Efficient Utilization of Soil and Fertilizer Resources, College of Resources and Environment, Shandong Agricultural University, Tai' an, 271018, China. yanhonglou1985@163.com.
Yuping ZhugeNational Engineering Research Center for Efficient Utilization of Soil and Fertilizer Resources, College of Resources and Environment, Shandong Agricultural University, Tai' an, 271018, China. zhugeyp@sdau.edu.cn.

Funding

the Key R&D Program of Shandong Province, China (2025CXPT151)
6 · The paper itself

Abstract

backgroundSoil salinity severely hampers early establishment of alfalfa (Medicago sativa L.) by inhibiting growth; impairing root system architecture (RSA) and aggravating oxidative, osmotic and membrane damage. This study aimed to investigate the individual and combined effects of two plant growth regulators-melatonin (MT) and 24-epibrassinolide (EBL)-on salt stress tolerance in alfalfa seedlings grown in coastal saline soil.

resultsWhen applied individually, EBL primarily restored establishment-related structural traits, with the best EBL dose (300 µg L

conclusionsMT and EBL jointly improved salt tolerance in alfalfa seedlings, but their main effects differed. EBL mainly supported growth recovery and root architectural rebuilding, whereas MT contributed more to redox balance, osmotic adjustment and membrane stability. Only traits with significant MT × EBL terms were treated as interaction-dependent responses; other combined improvements were interpreted as additive or complementary. These findings suggest that combined MT and EBL application may help improve alfalfa seedling establishment in salt-affected soils.

Indexed as

BrassinosteroidsMedicago sativaMelatoninPlant Growth RegulatorsSalt ToleranceSteroids, HeterocyclicAntioxidantsPlant RootsSalt StressSeedlingsAntioxidantsbrassinolideBrassinosteroidsMelatoninPlant Growth RegulatorsSteroids, Heterocyclic24-epibrassinolideAntioxidant enzymesMedicago sativa L.MelatoninMembrane stabilityRoot system architectureSalt stress

Identifiers

PMID42410340
PMCPMC13621765

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.