Evidence map›Paper›PMID 42406597›Full record

ArticleSTAR protocols2026

Protocol for isolation of live tumor-infiltrating immune cells from immunocompetent murine brains for high-dimensional profiling.

Aimée du Chatinier, Leyla Rigamonti, Piotr Waranecki, Dennis S Metselaar, Michaël H Meel, Senna Visser, Simone Foderaro, Serena R Baglio, Eelco W Hoving, Esther Hulleman

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Aimée du ChatinierPrincess Máxima Center for Pediatric Oncology, Utrecht 3584 CS, the Netherlands. Electronic address: a.duchatinier@prinsesmaximacentrum.nl.
Leyla RigamontiPrincess Máxima Center for Pediatric Oncology, Utrecht 3584 CS, the Netherlands.
Piotr WaraneckiPrincess Máxima Center for Pediatric Oncology, Utrecht 3584 CS, the Netherlands.
Dennis S MetselaarPrincess Máxima Center for Pediatric Oncology, Utrecht 3584 CS, the Netherlands.
Michaël H MeelPrincess Máxima Center for Pediatric Oncology, Utrecht 3584 CS, the Netherlands.
Senna VisserPrincess Máxima Center for Pediatric Oncology, Utrecht 3584 CS, the Netherlands.
Simone FoderaroAmsterdam UMC, Vrije Universiteit Amsterdam, Amsterdam HV 1081, the Netherlands.
Serena R BaglioAmsterdam UMC, Vrije Universiteit Amsterdam, Amsterdam HV 1081, the Netherlands.
Eelco W HovingPrincess Máxima Center for Pediatric Oncology, Utrecht 3584 CS, the Netherlands.
Esther HullemanPrincess Máxima Center for Pediatric Oncology, Utrecht 3584 CS, the Netherlands; Amsterdam UMC, Vrije Universiteit Amsterdam, Amsterdam HV 1081, the Netherlands. Electronic address: e.hulleman@prinsesmaximacentrum.nl.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Here, we present an optimized workflow to isolate viable immune cells from tumor-bearing mouse brains, with particular emphasis on myeloid populations, to enable detailed functional analysis of the tumor-immune microenvironment. We describe steps for intracardiac perfusion and regional brain dissection. We then detail procedures for gentle enzymatic-mechanical dissociation, efficient myelin and red blood cell removal, and optional CD11b

Indexed as

CancerImmunologyModel OrganismsNeuroscience

Identifiers

PMID42406597
PMCPMC13355811

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.