Evidence map›Paper›PMID 42404949›Full record

ArticleFrontiers in public health2026

Integrative assessment of species-level genetic markers for the diagnosis of pathogenic

Luiza Aymée, Vinicius de Carvalho Moura, Myllena Dos Santos Pereira, Walter Lilenbaum, Maria Isabel Nogueira Di Azevedo

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Article in Frontiers in public health, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Luiza AyméeLaboratory of Veterinary Bacteriology, Biomedical Institute of Fluminense Federal University, Niteroi, Brazil.
Vinicius de Carvalho MouraLaboratory of Investigation in Medical Microbiology, Institute of Microbiology, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
Myllena Dos Santos PereiraLaboratory of Investigation in Medical Microbiology, Institute of Microbiology, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
Walter LilenbaumLaboratory of Veterinary Bacteriology, Biomedical Institute of Fluminense Federal University, Niteroi, Brazil.
Maria Isabel Nogueira Di AzevedoLaboratory of Investigation in Medical Microbiology, Institute of Microbiology, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Leptospiral isolation and whole-genome sequencing are often unfeasible for genotyping; therefore, single-locus culture-independent sequencing is widely used. However, primer sets remain broadly applied without comprehensive quantitative or qualitative performance evaluation. Objective: This study integrated Methods: An integrative analysis evaluated 10 primer pairs targeting seven loci: Results: Only five markers showed good theoretical amplification performance: 16S rRNA, Discussion: The present study extends barcoding gap analysis to bacterial marker evaluation within an integrative framework, an approach previously applied mainly to Metazoa. It also highlights an important limitation in the widespread use of genotyping markers that lack reliable

Indexed as

LeptospiraLeptospirosisGenetic MarkersHumansPhylogenyRNA, Ribosomal, 16SSpecies SpecificityGenetic MarkersRNA, Ribosomal, 16Sbarcoding gapBLASTgenetic markerleptospirosisphylogenetics

Identifiers

PMID42404949
PMCPMC13328341

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.