Evidence map›Paper›PMID 42401933›Full record

ArticleBMC veterinary research2026

Duplex MIRA assays for simultaneous detection of novel goose astrovirus and goose circovirus.

Xiaomiao Hu, Huiqin Zhou, Yueyi Zhong, Fangfang Chen, Jieru Wang, Dongdong Yin, Lei Yin, Xuehuai Shen, Yayun Liu, Liyuan Chen and 4 more

Abstract read
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Article in BMC veterinary research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Xiaomiao Hu *Anhui Provincial Key Laboratory of Livestock and Poultry Product Safety, Livestock and Poultry Epidemic Diseases Research Center of Anhui Province, Institute of Animal Husbandry and Veterinary Science, Anhui Academy of Agricultural Science, Hefei, 230031, China.
Huiqin Zhou *College of Veterinary Medicine, Anhui Agricultural University, Hefei, 230036, China.
Yueyi ZhongCollege of Veterinary Medicine, Anhui Agricultural University, Hefei, 230036, China.
Fangfang ChenCollege of Veterinary Medicine, Anhui Agricultural University, Hefei, 230036, China.
Jieru WangAnhui Provincial Key Laboratory of Livestock and Poultry Product Safety, Livestock and Poultry Epidemic Diseases Research Center of Anhui Province, Institute of Animal Husbandry and Veterinary Science, Anhui Academy of Agricultural Science, Hefei, 230031, China.
Dongdong YinAnhui Provincial Key Laboratory of Livestock and Poultry Product Safety, Livestock and Poultry Epidemic Diseases Research Center of Anhui Province, Institute of Animal Husbandry and Veterinary Science, Anhui Academy of Agricultural Science, Hefei, 230031, China.
Lei YinAnhui Provincial Key Laboratory of Livestock and Poultry Product Safety, Livestock and Poultry Epidemic Diseases Research Center of Anhui Province, Institute of Animal Husbandry and Veterinary Science, Anhui Academy of Agricultural Science, Hefei, 230031, China.
Xuehuai ShenAnhui Provincial Key Laboratory of Livestock and Poultry Product Safety, Livestock and Poultry Epidemic Diseases Research Center of Anhui Province, Institute of Animal Husbandry and Veterinary Science, Anhui Academy of Agricultural Science, Hefei, 230031, China.
Yayun LiuAnhui Provincial Key Laboratory of Livestock and Poultry Product Safety, Livestock and Poultry Epidemic Diseases Research Center of Anhui Province, Institute of Animal Husbandry and Veterinary Science, Anhui Academy of Agricultural Science, Hefei, 230031, China.
Liyuan ChenAnhui Provincial Key Laboratory of Livestock and Poultry Product Safety, Livestock and Poultry Epidemic Diseases Research Center of Anhui Province, Institute of Animal Husbandry and Veterinary Science, Anhui Academy of Agricultural Science, Hefei, 230031, China.
Xiaocheng PanAnhui Provincial Key Laboratory of Livestock and Poultry Product Safety, Livestock and Poultry Epidemic Diseases Research Center of Anhui Province, Institute of Animal Husbandry and Veterinary Science, Anhui Academy of Agricultural Science, Hefei, 230031, China.
Lunzhi XiaAnhui Provincial Key Laboratory of Livestock and Poultry Product Safety, Livestock and Poultry Epidemic Diseases Research Center of Anhui Province, Institute of Animal Husbandry and Veterinary Science, Anhui Academy of Agricultural Science, Hefei, 230031, China. 939904965@qq.com.
Ruihong ZhaoAnhui Provincial Key Laboratory of Livestock and Poultry Product Safety, Livestock and Poultry Epidemic Diseases Research Center of Anhui Province, Institute of Animal Husbandry and Veterinary Science, Anhui Academy of Agricultural Science, Hefei, 230031, China. zrhkdy03@126.com.
Yin DaiAnhui Provincial Key Laboratory of Livestock and Poultry Product Safety, Livestock and Poultry Epidemic Diseases Research Center of Anhui Province, Institute of Animal Husbandry and Veterinary Science, Anhui Academy of Agricultural Science, Hefei, 230031, China. daiyin2020@163.com.

Funding

the Major Science and Technology Special Project in Anhui Province 202003a06020012
6 · The paper itself

Abstract

backgroundNovel goose astrovirus (NGAstV) and goose circovirus (GoCV) are two major pathogens responsible for disease outbreaks in goslings, causing substantial economic losses to the goose farming industry. In this study, two duplex multienzyme isothermal rapid amplification (MIRA) assays were developed for the simultaneous detection of NGAstV and GoCV, and the specificity and sensitivity of these detection methods were evaluated.

resultsBoth MIRA assays demonstrated high specificity for NGAstV and GoCV, with no cross-reactivity observed with six waterfowl pathogens, including duck enteritis virus, goose parvovirus, fowl adenovirus serotype 4, H9 subtype avian influenza virus, Muscovy duck reovirus and duck Tembusu virus. The basic duplex MIRA assay completed amplification within 25 min under a constant temperature of 25 °C, with minimum detection limits of 1 × 10

conclusionsThis method significantly improves pathogen detection efficiency and serves as an effective tool for the rapid identification of NGAstV and GoCV.

Indexed as

Astroviridae InfectionsAvastrovirusCircoviridae InfectionsCircovirusGeeseNucleic Acid Amplification TechniquesPoultry DiseasesAnimalsMolecular Diagnostic TechniquesRapid Diagnostic TestsSensitivity and SpecificityGoose circovirusMultienzyme isothermal rapid amplificationNovel goose astrovirusSimultaneous detection

Identifiers

PMID42401933
PMCPMC13637121

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.