Evidence map›Paper›PMID 42401592›Full record

ArticleScientific reports2026

Munc18-1 is crucial for photoreceptor function, survival and regulation of syntaxin-3 localization and expression.

Mengjia Huang, Chun Hin Chow, Ryan Noworolski, Jason Charish, Karen Indrawinata, Hidekiyo Harada, Valerie A Wallace, Philippe P Monnier, Shuzo Sugita

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In one paragraph

Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Mengjia Huang *Division of Experimental and Translational Neuroscience, Krembil Brain Institute, University Health Network, Toronto, ON, M5T 0S8, Canada.
Chun Hin Chow *Division of Experimental and Translational Neuroscience, Krembil Brain Institute, University Health Network, Toronto, ON, M5T 0S8, Canada.
Ryan Noworolski *Division of Experimental and Translational Neuroscience, Krembil Brain Institute, University Health Network, Toronto, ON, M5T 0S8, Canada.
Jason CharishDonald K. Johnson Eye Institute, University Health Network, Toronto, ON, M5T 0S8, Canada.
Karen IndrawinataDivision of Experimental and Translational Neuroscience, Krembil Brain Institute, University Health Network, Toronto, ON, M5T 0S8, Canada.
Hidekiyo HaradaDonald K. Johnson Eye Institute, University Health Network, Toronto, ON, M5T 0S8, Canada.
Valerie A WallaceDonald K. Johnson Eye Institute, University Health Network, Toronto, ON, M5T 0S8, Canada.
Philippe P MonnierDepartment of Physiology, Temerty Faculty of Medicine, University of Toronto, Toronto, ON, M5S 1A8, Canada.
Shuzo SugitaDivision of Experimental and Translational Neuroscience, Krembil Brain Institute, University Health Network, Toronto, ON, M5T 0S8, Canada. Shuzo.Sugita@uhn.ca.

Funding

CIHR PJT 165917Natural Sciences and Engineering Research Council of Canada RGPIN 2020 07137
6 · The paper itself

Abstract

The function of the SNARE complex regulator, Munc18-1, in photoreceptor cells is unknown. Here, we found that removing Munc18-1 from photoreceptors results in major degeneration starting at P14. In the absence of Munc18-1, before major photoreceptor degeneration, functional and synaptic impairments were present, indicating a critical function of Munc18-1. Furthermore, Munc18-1 played a critical role in expression and localization of syntaxin-3. The syntaxin-3 protein level is dramatically reduced in the soma and plasma membrane of photoreceptors without Munc18-1. At the photoreceptor synapses, the colocalization of syntaxin-3 and its SNARE partner, SNAP-25, was reduced, potentially suggesting an altered syntaxin-3 synaptic localization. In the Munc18-1-deficient photoreceptors, immature synapses and outer segment lesions were found. Taken together, these findings provide evidence that Munc18-1 is important for maintaining sufficient syntaxin-3 expression in the cell body and synapses of photoreceptors. The lack of Munc18-1, combined with poor syntaxin-3 expression, contributes to photoreceptor functional impairment and degeneration.

Identifiers

PMID42401592
PMCPMC13634923

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.