Protocol for ultrasound-guided injection into the murine portal vein to initiate liver metastasis.

Nicole Henning, Fred Roberts, Virginia Spanoudaki

Abstract read

In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Nicole HenningPreclinical Imaging and Testing Facility, Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02142, USA. Electronic address: nhenning@mit.edu.

Fred RobertsEMIT Imaging, Baltimore, MD, 21201, USA Formerly of Fujifilm VisualSonics Inc., Toronto, ON M4N 3N1, Canada.

Virginia SpanoudakiPreclinical Imaging and Testing Facility, Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02142, USA. Electronic address: vspan@mit.edu.

Funding

VIRUS PRODUCTION COREP30CA014051 · NCI · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI Paula T Hammond · 1985 to 2026

$93.9M

NCI NIH HHS P30 CA014051

6 · The paper itself

Abstract

Here, we present a non-surgical protocol for modeling murine liver metastasis by disseminating injected cells or compounds into the murine portal vein. We describe steps for identifying the portal vein and other vital structures adjacent to and within the liver by ultrasound, determining an optimal route to avoid vital structures, and injecting into the portal vein. We also report on the findings for reproducibility, adverse effects, and histopathological findings.

Indexed as

Biotechnology and bioengineeringCancerModel OrganismsOrganoids

Identifiers

PMID42400912

DOI10.1016/j.xpro.2026.104670

PMCPMC13355672

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.