ArticleMethods in molecular biology (Clifton, N.J.)2026
Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Programmable bacterial adhesion enables applications in biocatalysis, living materials, and targeted drug delivery. This functionality can be achieved through the expression of synthetic adhesins in bacterial cells. These molecules are hybrid proteins composed of four key elements: (i) a signal peptide for secretion; (ii) the beta barrel domain of an autotransporter for surface display; (iii) a nanobody module providing specific recognition and targeting capabilities; and (iv) an epitope tag for expression tracking and localization. This chapter describes a detailed protocol for visualizing these synthetic adhesins on Escherichia coli and Pseudomonas putida using indirect immunofluorescence combined with Super-Resolution Microscopy (SRM) techniques, such as STimulated Emission Depletion (STED). The workflow includes plasmid introduction into the bacterial cells, induction, immunostaining, and image acquisition to spatially track the adhesins on the cell surface.
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