Evidence map›Paper›PMID 42400842›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

De Novo Discovery of Prenylated Macrocyclic Peptide Ligands.

Sota Uchida, Hiroaki Suga, Yuki Goto

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Sota UchidaDepartment of Chemistry, Graduate School of Science, Kyoto University, Sakyo, Kyoto, Japan.
Hiroaki SugaDepartment of Chemistry, Graduate School of Science, The University of Tokyo, Bunkyo, Tokyo, Japan. hsuga@chem.s.u-tokyo.ac.jp.
Yuki GotoDepartment of Chemistry, Graduate School of Science, Kyoto University, Sakyo, Kyoto, Japan. goto.yuki.4x@kyoto-u.ac.jp.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Prenylated peptides, endowed with hydrophobic prenyl groups, are widely found as secondary metabolites in nature. To develop de novo artificial prenylated peptide ligands with selective affinity for a target protein of choice, a random prenylated peptide library can be constructed through the combination of a custom-made in vitro translation system and a peptide prenyltransferase, which is subsequently applied to mRNA display screening. In this chapter, we describe protocols for constructing a prenylated thioether-closed macrocyclic peptide library and conducting mRNA display screening to identify selective ligands.

Indexed as

Peptide LibraryPeptidesPeptides, CyclicLigandsPrenylationProtein BiosynthesisRNA, MessengerLigandsPeptide LibraryPeptidesPeptides, CyclicRNA, MessengerFIT systemIn vitro selectionMacrocyclic peptidemRNA displayPrenylationPseudo natural product peptidesRaPID systemRiPPsTranslation

Identifiers

PMID42400842

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.