Evidence map›Paper›PMID 42400835›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Generation of a CDR H3 Randomized Monoclonal Antibody Library by Kunkel Mutagenesis.

Jia Xuan Yeoh, Yee Siew Choong, Theam Soon Lim

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Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Jia Xuan YeohInstitute for Research in Molecular Medicine, Universiti Sains Malaysia, Penang, Malaysia.
Yee Siew ChoongInstitute for Research in Molecular Medicine, Universiti Sains Malaysia, Penang, Malaysia.
Theam Soon LimInstitute for Research in Molecular Medicine, Universiti Sains Malaysia, Penang, Malaysia. theamsoon@usm.my.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Complementary-determining regions (CDRs) are the primary factor that determines antibody diversity, while CDR H3 plays a central role in antigen binding. Randomization of this loop within a stable framework provides a powerful strategy for generating synthetic antibody libraries. This chapter outlines an approach that is Kunkel mutagenesis based, for constructing a monoclonal antibody library diversified at CDR H3. The method uses uracilated single-stranded DNA templates and degenerate oligonucleotides to introduce targeted variability while suppressing nonrecombinant background through strategically placed stop codons and restriction sites. The resulting repertoire is directly compatible with phage display for downstream selection. This streamlined protocol enables the generation of antibody libraries suitable for applications in affinity maturation, binder discovery, and antibody development.

Indexed as

Antibodies, MonoclonalComplementarity Determining RegionsMutagenesisPeptide LibraryCell Surface Display TechniquesHumansAntibodies, MonoclonalComplementarity Determining RegionsPeptide LibraryAffinity maturationAntibody librariesKunkel mutagenesisMonoclonal antibodyPhage display

Identifiers

PMID42400835

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.