ArticleMethods in molecular biology (Clifton, N.J.)2026
Ribosome RNA Extraction and RNase R Treatment.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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3 authors.
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Abstract
Chimeric RNAs can exert biological functions in various ways, and their biological functions often depend on their RNA sequences or their potential protein-coding ability. Here, we introduce two relatively mature experimental methods. One method is to extract ribosomes followed by PCR to determine whether the chimeric RNA has the ability to bind to ribosomes, thereby preliminarily evaluating its potential to code for proteins. The other method is to verify whether the chimeric RNA is resistant to RNase R, thereby preliminarily evaluating whether it has a circular RNA structure.
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