Untreated Rosehip Powder/Poly(Lactic Acid)/Poly(3-Hydroxybutyrate-Co-4-Hydroxybutyrate) Electrospun Mats for Wound Dressing Applications.

This study investigated the effects of directly incorporating untreated rosehip (RoHi) powder into electrospun poly(lactic acid)/poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (PLA/P(3HB-co-4HB)) (70/30, w/w) blends, focusing on the structural, morphological, mechanical, wettability, and biological properties of resulting nanofibrous mats. The average fiber diameter of pure nanofibers was 776 nm, while RoHi addition resulted in diameters ranging from 468 nm (1% RoHi) to 1496 nm (5% RoHi). FTIR analysis confirmed that the characteristic functional groups of RoHi were preserved within the polymer matrix without chemical interaction. The water contact angle decreased from 136.45° for the pure mat to 116.11° for nanofibers containing 5% RoHi, leading to an increase in liquid adsorption capacity from 1.49% to 249.38%. Cytotoxicity results demonstrated that all samples were biocompatible, with the highest cell viability (148.91%) observed for nanofibers containing 1% RoHi. In vitro scratch assays revealed enhanced fibroblast migration for RoHi-containing nanofibers, particularly within the first 24 h, with the fastest wound closure observed at 3% and 5% RoHi contents. DPPH analysis showed increased antioxidant activity from 27.68% to 63.84%, whereas no antibacterial activity was detected against Staphylococcus aureus and Pseudomonas aeruginosa. Overall, RoHi-doped nanofibers exhibit promising potential as wound dressing materials considered suitable for use in chronic wounds due to their antioxidant activity and bio-responsive wound-healing performance.