ArticleMolecular biology reports2026
Trametes versicolor laccase induces apoptosis and DNA damage in colorectal cancer via reactive oxygen species-mediated signaling disruption.
Article in Molecular biology reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
objectiveCopper-containing laccases oxidize various phenolic and non-phenolic compounds. Among them, fungal laccases have been extensively studied in industry, but their biomedical potential remains largely unexplored. We aimed to investigate the anticancer activity of the laccase enzyme, which we purified from Trametes versicolor without loss of activity using the three-phase partitioning (TPP) technique-an easy, inexpensive method that allows high activity recovery-on colorectal cancer (CRC) cells for the first time and to elucidate its mechanism of action.
methodsThe effects of the pure laccase enzyme were investigated on two CRC cell lines (HCT-116 and Caco-2) and normal endothelial cells (HUVEC). Cytotoxicity was assessed using the WST-1 assay. Apoptosis and cell cycle progression were analyzed by flow cytometry. Genotoxicity was determined by using micronucleus and comet assays. Intracellular DCFH-DA fluorescence intensity was measured, and the expression of apoptosis- and DNA repair-related genes was analyzed by qRT-PCR. The MAPK (p38, JNK) and NF-κB signaling pathways were examined by immunofluorescence.
resultsLaccase treatment exhibited a dose-dependent cytotoxic effect on CRC cells, with minimal impact on HUVECs. It induced apoptosis, oxidative stress-associated responses, DNA damage, and G0/G1 cell cycle arrest. Gene expression analysis revealed upregulation of Bax and Caspase-3 and downregulation of Bcl-2, RAD51, and NF-κB. Immunofluorescence confirmed activation of p38/JNK and suppression of NF-κB signaling.
conclusionTPP-purified fungal laccase exhibits selective cytotoxicity and pro-apoptotic effects in CRC cells via redox-mediated DNA damage and modulation of signaling pathways, supporting its potential as a novel anticancer agent.
Indexed as
Identifiers
42397491What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.