Evidence map›Paper›PMID 42395904›Full record

ArticleFrontiers in microbiology2026

Evaluation of nucleic acid-based matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for rapid identification of

Jiajun Zhou, Xiaoqing Lin, Yunan Zhang, Liufeng Qiu, Saiduo Liu, Zhengxing Wu, Yueying Zhou, Ning Pan, Chaochao Qiu, Jichan Shi

Abstract read
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In one paragraph

Article in Frontiers in microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Jiajun Zhou *Department of Infection, Wenzhou Central Hospital, Dingli Clinical College of Wenzhou Medical University, Wenzhou, Zhejiang, China.
Xiaoqing Lin *Department of Infection, Wenzhou Central Hospital, Dingli Clinical College of Wenzhou Medical University, Wenzhou, Zhejiang, China.
Yunan ZhangShanghai University, Shanghai, China.
Liufeng QiuDepartment of Infection, Wenzhou Central Hospital, Postgraduate Training Base Alliance of Wenzhou Medical University, Wenzhou, Zhejiang, China.
Saiduo LiuDepartment of Infection, Wenzhou Central Hospital, Dingli Clinical College of Wenzhou Medical University, Wenzhou, Zhejiang, China.
Zhengxing WuDepartment of Infection, Wenzhou Central Hospital, Dingli Clinical College of Wenzhou Medical University, Wenzhou, Zhejiang, China.
Yueying ZhouDepartment of Infection, Wenzhou Central Hospital, Postgraduate Training Base Alliance of Wenzhou Medical University, Wenzhou, Zhejiang, China.
Ning PanDepartment of Infection, Wenzhou Central Hospital, Postgraduate Training Base Alliance of Wenzhou Medical University, Wenzhou, Zhejiang, China.
Chaochao QiuDepartment of Infection, Wenzhou Central Hospital, Dingli Clinical College of Wenzhou Medical University, Wenzhou, Zhejiang, China.
Jichan ShiDepartment of Infection, Wenzhou Central Hospital, Dingli Clinical College of Wenzhou Medical University, Wenzhou, Zhejiang, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Objective: This study aimed to evaluate the value of nucleic acid-based matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) in identifying mycobacterial species and detecting drug resistance in patients with retreatment pulmonary tuberculosis (TB). Methods: The clinical data of 100 patients with retreatment pulmonary TB treated at Wenzhou Central Hospital between 1 June 2022 and 31 December 2023 were retrospectively collected and analyzed. Respiratory secretions were examined using BACTEC MGIT 960 Rapid Liquid Culture (MGIT 960 culture), drug-sensitive gene chip strain identification and nucleic acid-based MALDI-TOF MS assay (MassARRAY® system). Unlike traditional protein-based MALDI-TOF MS, this nucleic acid-based approach detects specific gene mutations associated with drug resistance. The results of the MGIT 960 culture and drug resistance testing served as the benchmark for evaluating the sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of the MALDI-TOF MS assay. Results: For Conclusion: The nucleic acid-based MALDI-TOF MS assay demonstrates promising performance in rapidly identifying MTB and detecting drug resistance, serving as a valuable complementary tool for rapid clinical diagnosis and personalized medication guidance in patients with retreatment TB. The detection of non-tuberculous mycobacteria requires further validation with larger sample sizes.

Indexed as

drug resistancematrix-assisted laser desorption ionization time-of-flight mass spectrometryMycobacterium tuberculosisnucleic acid detectionpulmonaryretreatment failuretuberculosis

Identifiers

PMID42395904
PMCPMC13323708

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