Evidence map›Paper›PMID 42393807›Full record

ArticleParasites & vectors2026

A novel activation-related gene P158 is essential for evagination and early development of Echinococcus multilocularis protoscoleces.

Zhendong Xin, Zhen Wang, Yong Fu, Chenye Jin, Xinyi Zhou, Yihui Wu, Wenjing Zhi, Mengjie Chu, Lijuan Zheng, Anni Zhang and 5 more

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Article in Parasites & vectors, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

15 authors.

Zhendong XinKey Laboratory of Animal Disease Diagnostics and Immunology, Ministry of Agriculture, MOE International Joint Collaborative Research Laboratory for Animal Health & Food Safety, Nanjing Agricultural University, Nanjing, China.
Zhen WangKey Laboratory of Animal Disease Diagnostics and Immunology, Ministry of Agriculture, MOE International Joint Collaborative Research Laboratory for Animal Health & Food Safety, Nanjing Agricultural University, Nanjing, China.
Yong FuQinghai Provincial Key Laboratory of Pathogen Diagnosis for Animal Diseases and Green Technical Research for Prevention and Control, Academy of Animal Science and Veterinary, Qinghai University, Xining, China.
Chenye JinKey Laboratory of Animal Disease Diagnostics and Immunology, Ministry of Agriculture, MOE International Joint Collaborative Research Laboratory for Animal Health & Food Safety, Nanjing Agricultural University, Nanjing, China.
Xinyi ZhouKey Laboratory of Animal Disease Diagnostics and Immunology, Ministry of Agriculture, MOE International Joint Collaborative Research Laboratory for Animal Health & Food Safety, Nanjing Agricultural University, Nanjing, China.
Yihui WuKey Laboratory of Animal Disease Diagnostics and Immunology, Ministry of Agriculture, MOE International Joint Collaborative Research Laboratory for Animal Health & Food Safety, Nanjing Agricultural University, Nanjing, China.
Wenjing ZhiKey Laboratory of Animal Disease Diagnostics and Immunology, Ministry of Agriculture, MOE International Joint Collaborative Research Laboratory for Animal Health & Food Safety, Nanjing Agricultural University, Nanjing, China.
Mengjie ChuKey Laboratory of Animal Disease Diagnostics and Immunology, Ministry of Agriculture, MOE International Joint Collaborative Research Laboratory for Animal Health & Food Safety, Nanjing Agricultural University, Nanjing, China.
Lijuan ZhengKey Laboratory of Animal Disease Diagnostics and Immunology, Ministry of Agriculture, MOE International Joint Collaborative Research Laboratory for Animal Health & Food Safety, Nanjing Agricultural University, Nanjing, China.
Anni ZhangKey Laboratory of Animal Disease Diagnostics and Immunology, Ministry of Agriculture, MOE International Joint Collaborative Research Laboratory for Animal Health & Food Safety, Nanjing Agricultural University, Nanjing, China.
Changjie QianKey Laboratory of Animal Disease Diagnostics and Immunology, Ministry of Agriculture, MOE International Joint Collaborative Research Laboratory for Animal Health & Food Safety, Nanjing Agricultural University, Nanjing, China.
Nariaki NonakaLaboratory of Parasitology, Department of Disease Control, Graduate School of Infectious Diseases, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Ryo NakaoLaboratory of Parasitology, Department of Disease Control, Graduate School of Infectious Diseases, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Zhihong GuoQinghai Provincial Key Laboratory of Pathogen Diagnosis for Animal Diseases and Green Technical Research for Prevention and Control, Academy of Animal Science and Veterinary, Qinghai University, Xining, China. 1984990033@qhu.edu.cn.
Gongguan LiuKey Laboratory of Animal Disease Diagnostics and Immunology, Ministry of Agriculture, MOE International Joint Collaborative Research Laboratory for Animal Health & Food Safety, Nanjing Agricultural University, Nanjing, China. gliu21@njau.edu.cn.

Funding

National Key Research and Development Program of China 2022YFE0108100
6 · The paper itself

Abstract

backgroundAlveolar echinococcosis is a lethal zoonotic parasitic disease caused by Echinococcus multilocularis. The activation and subsequent evagination of protoscoleces (PSCs) in the host digestive tract are critical steps for establishing infection in definitive hosts, yet the underlying molecular mechanisms remain poorly understood.

methodsIn this study, we employed an in vitro PSC activation model combined with transcriptomic analysis to identify activation-associated genes. A novel gene, designated P158, was further characterized by transcriptional unit analysis and functional knockdown assays to assess its role in PSC evagination, viability, tegument integrity, energy metabolism, and early metacestode development.

resultsWe confirmed that P158 constitutes an independent transcriptional unit from the adjacent DDX1 gene of E. multilocularis and found that P158 expression was significantly upregulated following activation. Knockdown of P158 led to a significant reduction in PSC evagination rate and impaired metacestode vesicular development in vitro. Concurrently, decreased cellular viability and damage to the microtriches indicated compromised tegument structural integrity. Further mechanistic analysis showed that P158 knockdown triggered metabolic dysregulation, characterized by reduced ATP levels, shrinkage of glycogen storage cells, and depletion of intracellular glycogen reserves.

conclusionsThese findings demonstrate that P158 plays an essential role in promoting PSC evagination and early development by maintaining tegument structural integrity and regulating energy metabolism homeostasis. This study highlights P158 as a potential target for interventions aimed at blocking parasite development at the definitive host stage.

Indexed as

Echinococcus multilocularisHelminth ProteinsAnimalsEchinococcosisEnergy MetabolismGene Expression ProfilingGene Knockdown TechniquesHelminth ProteinsActivation-associated geneEchinococcus multilocularisEvaginated protoscolexP158 geneProtoscolex activationRNA interference

Identifiers

PMID42393807
PMCPMC13599111

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.