Evidence map›Paper›PMID 42393358›Full record

ArticleNature cell biology2026

Cell surface liposome binding (CLiB) allows lipid-binding probe engineering via high-throughput screening.

Taki Nishimura, Kotaro Tsuboyama, Yuki Nakagaki, Shiou-Ling Lu, Yuki Ishino, Nozomu Kono, Takeshi Noda, Eiji Yamamoto, Noboru Mizushima

Abstract read
PubMed Publisher
In one paragraph

Article in Nature cell biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Taki NishimuraInstitute for Protein Research, The University of Osaka, Osaka, Japan. ntaki@protein.osaka-u.ac.jp.ORCID http://orcid.org/0000-0003-4019-5984
Kotaro TsuboyamaInstitute for Protein Research, The University of Osaka, Osaka, Japan. kotaro.tsuboyama@gmail.com.ORCID http://orcid.org/0000-0002-9034-8179
Yuki Nakagaki *Department of System Design Engineering, Keio University, Kanagawa, Japan.
Shiou-Ling Lu *Department of Oral Cellular Biology, Center for Frontier Oral Science, Graduate School of Dentistry, The University of Osaka, Osaka, Japan.ORCID http://orcid.org/0000-0003-4308-1536
Yuki IshinoLaboratory of Health Chemistry, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, Japan.ORCID http://orcid.org/0000-0001-6764-7405
Nozomu KonoLaboratory of Health Chemistry, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, Japan.ORCID http://orcid.org/0000-0002-0871-8477
Takeshi NodaGraduate School of Frontier Biosciences, The University of Osaka, Osaka, Japan.ORCID http://orcid.org/0000-0003-3581-7961
Eiji YamamotoDepartment of System Design Engineering, Keio University, Kanagawa, Japan.ORCID http://orcid.org/0000-0002-8813-5297
Noboru MizushimaDepartment of Biochemistry and Molecular Biology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.ORCID http://orcid.org/0000-0002-6258-6444

Funding

Japan Agency for Medical Research and Development (AMED) JP21gm1210013MEXT | Japan Science and Technology Agency (JST) JPMJFR226AMEXT | Japan Science and Technology Agency (JST) JPMJFR230ZMEXT | Japan Science and Technology Agency (JST) JPMJGX23B4MEXT | Japan Science and Technology Agency (JST) JPMJGX23B9MEXT | Japan Science and Technology Agency (JST) JPMJTR24U7MEXT | Japan Society for the Promotion of Science (JSPS) 21H05146MEXT | Japan Society for the Promotion of Science (JSPS) 22H04919MEXT | Japan Society for the Promotion of Science (JSPS) 22K06171MEXT | Japan Society for the Promotion of Science (JSPS) 23H02475MEXT | Japan Society for the Promotion of Science (JSPS) 24H01117MEXT | Japan Society for the Promotion of Science (JSPS) 24H01356MEXT | Japan Society for the Promotion of Science (JSPS) 24K02019MEXT | Japan Society for the Promotion of Science (JSPS) 25H02268MEXT | Japan Society for the Promotion of Science (JSPS) 25K09629MEXT | Japan Society for the Promotion of Science (JSPS) 26H01630MEXT | Japan Society for the Promotion of Science (JSPS) 26H01632MEXT | JST | Core Research for Evolutional Science and Technology (CREST) JPMJCR23B6MEXT | JST | Precursory Research for Embryonic Science and Technology (PRESTO) JPMJPR20ECMEXT | JST | Precursory Research for Embryonic Science and Technology (PRESTO) JPMJPR21E9MEXT | JST | Precursory Research for Embryonic Science and Technology (PRESTO) JPMJPR22EE
6 · The paper itself

Abstract

Lipid-binding domains, traditionally isolated from natural proteins, are essential tools for probing membrane lipid dynamics and specialized cellular compartments. Despite diverse applications, a general strategy for their engineering remains elusive. Here we present a robust and high-throughput method for monitoring protein-lipid interactions, named the cell surface liposome binding (CLiB) assay. Using the assay, we conducted directed evolution of the PX domain from SnxA, isolating high-affinity variants specific for phosphatidylinositol 3,5-bisphosphate (PI(3,5)P

Indexed as

Cell MembraneHigh-Throughput Screening AssaysLiposomesPhosphatidylinositol PhosphatesAnimalsDirected Molecular EvolutionHumansProtein BindingSaccharomyces cerevisiaeLiposomesphosphatidylinositol 3,5-diphosphatePhosphatidylinositol Phosphates

Identifiers

PMID42393358

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.