Evidence map›Paper›PMID 42392077›Full record

ArticleCell2026

A viral ORFeome library for systems-level genetic dissection of host-pathogen interactions.

Eric Fujimura, Colin N O'Leary, Mamie Z Li, Rachel A Roberts, Caleb R Glassman, Joao A Paulo, Hanjie Jiang, Nouran S Abdelfattah, Eric C Wooten, Zachary Mirman and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. The diverse functions of viral microproteins.bioRxiv : the preprint server for biology · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Eric FujimuraDepartment of Genetics, Harvard Medical School, Boston, MA 02115, USA; Division of Genetics, Department of Medicine, Brigham and Women's Hospital, Boston, MA 02115, USA; Program in Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02115, USA.
Colin N O'LearyDepartment of Genetics, Harvard Medical School, Boston, MA 02115, USA; Division of Genetics, Department of Medicine, Brigham and Women's Hospital, Boston, MA 02115, USA; Program in Virology, Harvard Medical School, Boston, MA 02115, USA.
Mamie Z LiDivision of Genetics, Department of Medicine, Brigham and Women's Hospital, Boston, MA 02115, USA; Howard Hughes Medical Institute, Boston, MA 02115, USA.
Rachel A RobertsDepartment of Genetics, Harvard Medical School, Boston, MA 02115, USA; Division of Genetics, Department of Medicine, Brigham and Women's Hospital, Boston, MA 02115, USA.
Caleb R GlassmanDepartment of Genetics, Harvard Medical School, Boston, MA 02115, USA; Division of Genetics, Department of Medicine, Brigham and Women's Hospital, Boston, MA 02115, USA.
Joao A PauloDepartment of Cell Biology, Harvard Medical School, Boston, MA 02115, USA.
Hanjie JiangDivision of Genetics, Department of Medicine, Brigham and Women's Hospital, Boston, MA 02115, USA; Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA.
Nouran S AbdelfattahDepartment of Genetics, Harvard Medical School, Boston, MA 02115, USA; Division of Genetics, Department of Medicine, Brigham and Women's Hospital, Boston, MA 02115, USA; Program in Biological and Biomedical Sciences, Harvard Medical School, Boston, MA 02115, USA.
Eric C WootenDepartment of Genetics, Harvard Medical School, Boston, MA 02115, USA; Division of Genetics, Department of Medicine, Brigham and Women's Hospital, Boston, MA 02115, USA.
Zachary MirmanDepartment of Genetics, Harvard Medical School, Boston, MA 02115, USA; Division of Genetics, Department of Medicine, Brigham and Women's Hospital, Boston, MA 02115, USA.
J Wade HarperDepartment of Cell Biology, Harvard Medical School, Boston, MA 02115, USA.
Philip A ColeDivision of Genetics, Department of Medicine, Brigham and Women's Hospital, Boston, MA 02115, USA; Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA.
Stephen J ElledgeDepartment of Genetics, Harvard Medical School, Boston, MA 02115, USA; Division of Genetics, Department of Medicine, Brigham and Women's Hospital, Boston, MA 02115, USA; Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA; Howard Hughes Medical Institute, Boston, MA 02115, USA. Electronic address: selledge@genetics.med.harvard.edu.

Funding

Ubiquitin Mediated Proteolysis and Cell Cycle ControlR01AG011085 · NIA · HARVARD UNIVERSITY (MEDICAL SCHOOL) · PI JEFFREY W HARPER · 1993 to 2026
$10.9M
Chemical Approaches to Protein PhosphorylationR01CA074305 · NCI · JOHNS HOPKINS UNIVERSITY · PI COLE, PHILIP A · 2002 to 2025
$8.0M
Genome Integrity Through the Cell Cycle in Homeostasis and CancerK00CA245720 · NCI · BRIGHAM AND WOMEN'S HOSPITAL · PI MIRMAN, ZACHARY KENNETH · 2022 to 2025
$397k
ENZYMATIC STUDIES ON PROTEIN TYROSINE KINASE CSKR29CA074305 · NCI · ROCKEFELLER UNIVERSITY · PI COLE, PHILIP A · 1997 to 2001
$188k
Bill & Melinda Gates Foundation OPP1155863NCI NIH HHS K00 CA245720NCI NIH HHS R01 CA074305NCI NIH HHS R29 CA074305NIA NIH HHS R01 AG011085
6 · The paper itself

Abstract

Virological research has traditionally focused on individual viruses or viral families. Advances in DNA synthesis now allow large-scale construction of individual gene products, enabling systematic exploration of the virome. Here, we developed a barcoded library of ∼12,000 viral open reading frames (vORFs) from 513 viral species, which we leveraged to identify hundreds of viral regulators of cellular proliferation, MHC class I antigen presentation, and interferon signaling. Integrating results across these screens revealed unique phenotypic profiles and functional vORF modules, allowing the in-depth characterization of two previously uncharacterized viral proteins, MC162R and Yaba-like disease virus (YLDV) 151R, which impair MHC class I antigen presentation and interferon (IFN)-β signaling, respectively. Together, the viral ORFeome provides a scalable framework for dissecting viral protein function across the breadth of the virome.

Indexed as

Host-Pathogen InteractionsOpen Reading FramesViromeVirusesAnimalsAntigen PresentationGene LibraryHumansInterferon-betaSignal TransductionViral ProteinsInterferon-betaViral Proteinsantigen presentationE3 ubiquitin ligasegenetic screensHost-virus interactionsimmune evasioninterferon signalingvirome

Identifiers

PMID42392077
PMCPMC13429030

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.