ArticleSTAR protocols2026
Protocol for inducing deep vein thrombosis in C57BL/6J mice using the inferior vena cava stenosis model.
Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Authors and funding
5 authors.
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Abstract
Here, we present a protocol for inducing deep vein thrombosis (DVT) in C57BL/6J mice using the inferior vena cava (IVC) stenosis model. We describe steps for surgical preparation, IVC ligation, and non-invasive detection and confirmation of thrombus development using high-frequency ultrasound (HFUS). We then detail procedures to dissect thrombi, prepare single-cell suspensions, and analyze infiltrating myeloid cells, including monocytes, by flow cytometry. This protocol allows precise timing, visualization, and immune profiling of early thrombus formation within 48 h. For complete details and execution of this protocol, please refer to Shahneh et al.
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