Evidence map›Paper›PMID 42391006›Full record

ArticleSTAR protocols2026

Protocol for longitudinal two-photon calcium imaging and holographic optogenetic manipulation to investigate memory in mice.

Huaiqin Guo, He Yang, Feng Su, Yuzhi Zhu, Qingyi Tang, Rui Cao, Qiao Wang, Siyi Tong, Shaoli Wang, Chen Zhang and 1 more

Abstract read
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In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Huaiqin GuoDepartment of Neurosurgery, Huashan Hospital, Institute for Translational Brain Research, State Key Laboratory of Brain Function and Disorders, MOE Frontiers Center for Brain Science, Fudan University, Shanghai, China.
He YangDepartment of Neurosurgery, Huashan Hospital, Institute for Translational Brain Research, State Key Laboratory of Brain Function and Disorders, MOE Frontiers Center for Brain Science, Fudan University, Shanghai, China.
Feng SuNational Biomedical Imaging Center, College of Future Technology, Peking University, Beijing, China.
Yuzhi ZhuDepartment of Neurosurgery, Huashan Hospital, Institute for Translational Brain Research, State Key Laboratory of Brain Function and Disorders, MOE Frontiers Center for Brain Science, Fudan University, Shanghai, China.
Qingyi TangDepartment of Neurosurgery, Huashan Hospital, Institute for Translational Brain Research, State Key Laboratory of Brain Function and Disorders, MOE Frontiers Center for Brain Science, Fudan University, Shanghai, China.
Rui CaoSchool of Information Science and Engineering and Yau Shing-Tung Center, Southeast University, Nanjing 210096, China.
Qiao WangSchool of Information Science and Engineering and Yau Shing-Tung Center, Southeast University, Nanjing 210096, China.
Siyi TongDepartment of Neurosurgery, Huashan Hospital, Institute for Translational Brain Research, State Key Laboratory of Brain Function and Disorders, MOE Frontiers Center for Brain Science, Fudan University, Shanghai, China.
Shaoli WangDepartment of Neurosurgery, Huashan Hospital, Institute for Translational Brain Research, State Key Laboratory of Brain Function and Disorders, MOE Frontiers Center for Brain Science, Fudan University, Shanghai, China. Electronic address: shaoliwang@fudan.edu.cn.
Chen ZhangSchool of Basic Medical Sciences, Laboratory of Organ Synergy and Intelligent Regenerative Manufacturing, Capital Medical University, Beijing 100069, China; College of Basic Medicine, Inner Mongolia Medical University, Hohhot 010110, China; State Key Laboratory of Translational Medicine and Innovative Drug Development, Nanjing, Jiangsu 210000, China. Electronic address: czhang@ccmu.edu.cn.
Wei LuDepartment of Neurosurgery, Huashan Hospital, Institute for Translational Brain Research, State Key Laboratory of Brain Function and Disorders, MOE Frontiers Center for Brain Science, Fudan University, Shanghai, China; MOE Innovative Center for New Drug Development of Immune Inflammatory Diseases, Fudan University, Shanghai, China; MOE Key Laboratory of Developmental Genes and Human Disease, School of Life Science and Technology, Southeast University, Nanjing, Jiangsu Province 210096, China. Electronic address: lu_wei@fudan.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Here, we present a protocol for longitudinal two-photon calcium imaging and holographic optogenetic manipulation to investigate memory in mice. We describe chronic cranial window implantation, stereotaxic viral delivery, and head-fixed contextual fear conditioning synchronized with imaging acquisition and optical stimulation of identified neurons. We further detail procedures for image data processing pipelines for cross-session registration and longitudinal tracking of the same neuronal population. This protocol enables simultaneous neuronal activity recording, behavioral monitoring, and causal interrogation of neuronal ensembles. For complete details on the use and execution of this protocol, please refer to Wang et al.

Indexed as

BehaviorCognitive NeuroscienceMicroscopy

Identifiers

PMID42391006
PMCPMC13352386

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.