Protocol for in vitro phase separation of ARID3B proteins.

Wenyu Han, Xiaofeng Li, Junyan Lin, Yongchu Pan

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Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

4 authors.

Wenyu HanDepartment of Orthodontics, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, China; State Key Laboratory Cultivation Base of Research, Prevention and Treatment for Oral Diseases (Nanjing Medical University), Nanjing, China; Jiangsu Province Engineering Research Center of Stomatological Translational Medicine (Nanjing Medical University), Nanjing, China.

Xiaofeng LiDepartment of Orthodontics, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, China; State Key Laboratory Cultivation Base of Research, Prevention and Treatment for Oral Diseases (Nanjing Medical University), Nanjing, China; Jiangsu Province Engineering Research Center of Stomatological Translational Medicine (Nanjing Medical University), Nanjing, China. Electronic address: xfli@stu.njmu.edu.cn.

Junyan LinDepartment of Orthodontics, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, China; State Key Laboratory Cultivation Base of Research, Prevention and Treatment for Oral Diseases (Nanjing Medical University), Nanjing, China; Jiangsu Province Engineering Research Center of Stomatological Translational Medicine (Nanjing Medical University), Nanjing, China.

Yongchu PanDepartment of Orthodontics, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, China; State Key Laboratory Cultivation Base of Research, Prevention and Treatment for Oral Diseases (Nanjing Medical University), Nanjing, China; Jiangsu Province Engineering Research Center of Stomatological Translational Medicine (Nanjing Medical University), Nanjing, China. Electronic address: panyongchu@njmu.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Liquid-liquid phase separation (LLPS) of ARID3B is critical in nonsyndromic cleft lip/palate pathogenesis. Here, we present a protocol to visualize ARID3B LLPS. We describe steps for regenerating LLPS with purified protein in vitro and detecting ARID3B condensates in mammalian cells. This protocol enables analysis of ARID3B phase separation dynamics and the study of other transcription factors that undergo LLPS. For complete details on the use and execution of this protocol, please refer to Li et al.

Indexed as

Cell BiologyGeneticsImmunologyMicroscopyMolecular BiologyProtein Biochemistry

Identifiers

PMID42391004

DOI10.1016/j.xpro.2026.104683

PMCPMC13355443

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.