ArticleMethods in molecular biology (Clifton, N.J.)2026
Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Chromosome conformation capture (3C), when combined with high-throughput next-generation sequencing, known as Hi-C, is a powerful approach for visualizing three-dimensional genome architecture. This technique enables detailed study of how chromosome topology influences gene regulation and other key cellular processes.Incorporating a sequence capture step into the Hi-C workflow enables targeted enrichment of predefined genomic regions of interest. Compared with conventional Hi-C, Capture Hi-C (CHi-C) increases the sequencing depth for the targeted regions, enabling high-resolution analysis of chromatin structure within a predefined locus at a lower cost.Here, we present a detailed protocol for CHi-C applied to a region of the Arabidopsis thaliana genome encompassing the thalianol biosynthetic gene cluster.
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