Evidence map›Paper›PMID 42390753›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Zhiyu Zhong, Lin Liu, Liang Deng, Zhihu Zhao, Yuhui Sun

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Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Zhiyu ZhongSchool of Pharmacy, Huazhong University of Science and Technology, Wuhan, China.
Lin LiuWuhan Frasergen Bioinformatics Co., Ltd., Wuhan, China.
Liang DengKey Laboratory of Combinatorial Biosynthesis and Drug Discovery (Ministry of Education), Wuhan University, Wuhan, China.
Zhihu ZhaoDepartment of Protein Engineering, Beijing Institute of Biotechnology, Beijing, China.
Yuhui SunSchool of Pharmacy, Huazhong University of Science and Technology, Wuhan, China. sunyuhui@hust.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Streptomyces species are prolific producers of bioactive compounds, including antibiotics, but many of their biosynthetic gene clusters (BGCs) remain silent or are poorly expressed under laboratory conditions. Recent studies have reported a close correlation between chromosomal organization and the expression activity of genes, including BGCs. Chromosome conformation capture with high-throughput sequencing (Hi-C) is a technique that allows for the profiling of three-dimensional genome organization by capturing physical interactions between chromosomal regions. In this chapter, we describe the application of Hi-C to Streptomyces coelicolor, focusing on the detailed protocol for constructing a Hi-C library and analyzing chromosomal interactions. By using this technique, researchers can explore the relationship between chromosomal architecture and the expression of BGCs, which may inform new strategies for improving natural product yields in Streptomyces.

Indexed as

Chromosome MappingChromosomes, BacterialHigh-Throughput Nucleotide SequencingStreptomycesStreptomyces coelicolorGene LibraryGenome, BacterialMultigene FamilyChromosomal organizationData analysisHi-CLibrary preparationStreptomyces

Identifiers

PMID42390753

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.