Evidence map›Paper›PMID 42390751›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Panagiotis Vletsos, Jenny Koukara, Kalliope K Papadopoulou

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Panagiotis VletsosLaboratory of Plant and Environmental Biotechnology, Department of Biochemistry and Biotechnology, University of Thessaly, Larissa, Greece.
Jenny KoukaraLaboratory of Plant and Environmental Biotechnology, Department of Biochemistry and Biotechnology, University of Thessaly, Larissa, Greece.
Kalliope K PapadopoulouLaboratory of Plant and Environmental Biotechnology, Department of Biochemistry and Biotechnology, University of Thessaly, Larissa, Greece. kalpapad@bio.uth.gr.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Rhizobium rhizogenes-mediated hairy root transformation has been extensively applied across various legumes, ranging from model species such as Medicago truncatula and Lotus japonicus to crop legumes like Glycine max and Phaseolus vulgaris. This technique is highly regarded for its simplicity and rapid production of transgenic roots, making it invaluable for studies on gene expression and function, root biology, symbiotic interactions, and metabolic engineering. Consequently, multiple protocols have been developed to optimize its use in legumes. In this chapter, we present a detailed protocol for hairy root transformation in the model plant Lotus japonicus, emphasizing key elements essential for success that can be adapted for use in other legumes.

Indexed as

FabaceaeLotusPlant RootsRhizobiumTransformation, GeneticAgrobacteriumPlants, Genetically ModifiedHairy root transformationLegumesLotus japonicusRhizobium rhizogenes

Identifiers

PMID42390751

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.