ArticleMicrobiology spectrum2026
Antifolate agent aminopterin demonstrates potent anti-monkeypox virus activity
Article in Microbiology spectrum, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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4 authors.
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Abstract
The ongoing spread of the monkeypox outbreak underscores the urgent need for the development of effective therapeutics against the monkeypox virus (MPXV). Aminopterin is a classical antifolate agent known for its anti-tumor activity through the inhibition of folate metabolism. Here, aminopterin was identified as an effective anti-MPXV agent. It significantly inhibited MPXV replication, unlike other antifolate agents such as methotrexate, pemetrexed, and LSN3213218. In Vero E6 cells, the half-maximal inhibitory concentration (IC₅₀) was 36.10 nM, the half-cytotoxic concentration (CC₅₀) was 3,310 nM, and the selectivity index (SI) was 91.690. In human foreskin fibroblast cells, the IC IMPORTANCE: The urgent need for effective monkeypox virus (MPXV) therapeutics is underscored by the ongoing outbreak. This study identifies aminopterin as a potent anti‑MPXV agent among four antifolate agents. Crucially, aminopterin suppresses viral infection and mitigates infection-associated inflammation
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