Evidence map›Paper›PMID 42389571›Full record

ArticleiScience2026

Toward point-of-care and amplification-free detection of human cytomegalovirus using CRISPR-Cas12a.

Kavish A V Kohabir, A W J Rietveld, Lars O Nooi, Roos E Beijer, Jeanne E van Dongen, Jasper Linthorst, Rob M F Wolthuis, Marcel Jonges, Matthijs R A Welkers, Loes I Segerink and 1 more

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Article in iScience, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Kavish A V KohabirDepartment of Human Genetics, Amsterdam UMC, Location Vrije Universiteit, Amsterdam, the Netherlands.
A W J RietveldBIOS Lab on a Chip Group, MESA Institute for Nanotechnology, Technical Medical Center, Max Planck Institute for Complex Fluid Dynamics, University of Twente, Enschede, the Netherlands.
Lars O NooiDepartment of Human Genetics, Amsterdam UMC, Location Vrije Universiteit, Amsterdam, the Netherlands.
Roos E BeijerDepartment of Human Genetics, Amsterdam UMC, Location Vrije Universiteit, Amsterdam, the Netherlands.
Jeanne E van DongenBIOS Lab on a Chip Group, MESA Institute for Nanotechnology, Technical Medical Center, Max Planck Institute for Complex Fluid Dynamics, University of Twente, Enschede, the Netherlands.
Jasper LinthorstDepartment of Human Genetics, Amsterdam UMC, Location Vrije Universiteit, Amsterdam, the Netherlands.
Rob M F WolthuisDepartment of Human Genetics, Amsterdam UMC, Location Vrije Universiteit, Amsterdam, the Netherlands.
Marcel JongesDepartment of Medical Microbiology and Infection Prevention, Amsterdam University Medical Centers, Amsterdam, the Netherlands.
Matthijs R A WelkersAmsterdam Institute for Immunology and Infectious Diseases, Amsterdam, the Netherlands.
Loes I SegerinkBIOS Lab on a Chip Group, MESA Institute for Nanotechnology, Technical Medical Center, Max Planck Institute for Complex Fluid Dynamics, University of Twente, Enschede, the Netherlands.
Erik A SistermansDepartment of Human Genetics, Amsterdam UMC, Location Vrije Universiteit, Amsterdam, the Netherlands.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Human cytomegalovirus (hCMV) is a herpesvirus that establishes lifelong latency in myeloid cells, posing health concerns particularly in fetal development and in immunocompromised individuals. Point-of-care (PoC) detection of hCMV DNA in liquid biopsies supports timely diagnosis and proper mitigation. However, ultra-low concentrations and high fragmentation rates, challenge primer-based preamplification methods. We present a proof-of-concept amplification-free CRISPR-based assay, exploiting the inherent specificity and signal-amplification of Cas12a and improving signal using a combinatorial approach. Optimizing Cas12a's

Indexed as

BioengineeringBiomedical engineeringBiotechnologyMicrobiologyMolecular biology

Identifiers

PMID42389571
PMCPMC13320335

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.