Evidence map›Paper›PMID 42385708›Full record

ArticleCell systems2026

High-throughput DNA engineering by mating bacteria.

Takeshi Matsui, Po-Hsiang Hung, Han Mei, Xianan Liu, Fangfei Li, John Collins, Weiyi Li, Darach Miller, Neil Wilson, Esteban Toro and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell systems, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

13 authors.

Takeshi MatsuiBacStitch DNA, Inc., Los Altos, CA, USA; SLAC National Accelerator Laboratory, Menlo Park, CA, USA.
Po-Hsiang HungBacStitch DNA, Inc., Los Altos, CA, USA; Stanford Department of Genetics, Palo Alto, CA, USA.
Han MeiBacStitch DNA, Inc., Los Altos, CA, USA; SLAC National Accelerator Laboratory, Menlo Park, CA, USA.
Xianan LiuSLAC National Accelerator Laboratory, Menlo Park, CA, USA; Twist Biosciences, Inc., South San Francisco, CA, USA.
Fangfei LiBacStitch DNA, Inc., Los Altos, CA, USA; Stanford Department of Genetics, Palo Alto, CA, USA.
John CollinsBacStitch DNA, Inc., Los Altos, CA, USA.
Weiyi LiSLAC National Accelerator Laboratory, Menlo Park, CA, USA; Stanford Department of Genetics, Palo Alto, CA, USA.
Darach MillerBacStitch DNA, Inc., Los Altos, CA, USA; SLAC National Accelerator Laboratory, Menlo Park, CA, USA.
Neil WilsonBacStitch DNA, Inc., Los Altos, CA, USA.
Esteban ToroTwist Biosciences, Inc., South San Francisco, CA, USA.
Geoffrey J TaghonNational Institute of Standards and Technology, Gaithersburg, MD, USA.
Gavin SherlockStanford Department of Genetics, Palo Alto, CA, USA.
Sasha LevyBacStitch DNA, Inc., Los Altos, CA, USA; SLAC National Accelerator Laboratory, Menlo Park, CA, USA. Electronic address: sasha@bacstitchdna.com.

Funding

Multiplexed In Vivo DNA AssemblyR01HG011676 · NHGRI · STANFORD UNIVERSITY · PI LEVY, SASHA F · 2022 to 2024
$2.9M
NHGRI NIH HHS R01 HG011676
6 · The paper itself

Abstract

We introduce SCRIVENER (sequential conjugation and recombination for in vivo elongation of nucleotides with low errors), an in vivo DNA assembly platform that streamlines and scales DNA engineering. SCRIVENER combines bacterial conjugation, in vivo DNA cutting, and homologous recombination to stitch DNA blocks together by mating E. coli in large arrays or pools. This approach is simpler, cheaper, and higher throughput than methods requiring DNA to be moved in and out of cells. We performed over 5,000 assemblies with 2 to 19 blocks (240 bp-12 kb) and assembled constructs up to 81 kb with high fidelity. Most errors are deletions between long repeats, but SCRIVENER minimizes their impact by enabling high-replication assembly and sequence verification at a nominal additional cost per replicate. The platform enables combinatorial library construction and DNA block reuse without PCR and is therefore a powerful tool to accelerate DNA design-build-test-learn cycles. A record of this paper's transparent peer review process is included in the supplemental information.

Indexed as

Escherichia coliGenetic EngineeringConjugation, GeneticDNADNA, BacterialHomologous RecombinationDNADNA, BacterialcomposabilityconjugationDNA assemblyDNA engineeringDNA reuseDNA synthesisgenetic toolshigh-throughputin vivoplasmid engineering

Identifiers

PMID42385708
PMCPMC13656942

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.