Protocol to prepare inclusion complex-enhanced cannabinoid nano-micelles for behavioral pharmacology and in vivo neurophysiology in mice.

Jingyu Feng, Jessica Page, Leeyup Chung, Zhigang He, Kuan Hong Wang

Abstract read

In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Jingyu FengDepartment of Neuroscience, Del Monte Institute for Neuroscience, University of Rochester Medical Center, Rochester, NY 14642, USA. Electronic address: jingyu_feng@urmc.rochester.edu.

Jessica PageF.M. Kirby Neurobiology Center, Boston Children's Hospital, and Department of Neurology and Ophthalmology, Harvard Medical School, Boston, MA 02115, USA.

Leeyup ChungF.M. Kirby Neurobiology Center, Boston Children's Hospital, and Department of Neurology and Ophthalmology, Harvard Medical School, Boston, MA 02115, USA.

Zhigang HeF.M. Kirby Neurobiology Center, Boston Children's Hospital, and Department of Neurology and Ophthalmology, Harvard Medical School, Boston, MA 02115, USA. Electronic address: zhigang.he@childrens.harvard.edu.

Kuan Hong WangDepartment of Neuroscience, Del Monte Institute for Neuroscience, University of Rochester Medical Center, Rochester, NY 14642, USA. Electronic address: kuanhong_wang@urmc.rochester.edu.

Funding

Mechanism and optimization of CBD-mediated analgesic effects (Diversity Supplement)R01AT010779 · NCCIH · BOSTON CHILDREN'S HOSPITAL · PI HE, ZHIGANG, WANG, KUAN HONG · 2019 to 2023

$4.3M

NCCIH NIH HHS R01 AT010779

6 · The paper itself

Abstract

Cannabinoids have significant therapeutic potential but are limited by hydrophobicity and low bioavailability. Here, we present a protocol for preparing a nano-micellar cannabidiol formulation (CBD-IN) using CBD, (2-hydroxypropyl)-β-cyclodextrin, and polysorbate 80. We describe the steps to produce 16-32 nm micelles that markedly enhance cannabinoid water solubility. We detail quality-control procedures using liquid chromatography-mass spectrometry and cryo-transmission electron microscopy. This protocol applies to real-time mouse behavioral pharmacology and in vivo neurophysiology studies through rapid systemic delivery and central nervous system exposure. For complete details on the use and execution of this protocol, please refer to Feng et al.

Indexed as

ChemistryHealth SciencesNeuroscience

Identifiers

PMID42384494

DOI10.1016/j.xpro.2026.104650

PMCPMC13352389

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LicenceCC BY-NC-ND

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.