Evidence map›Paper›PMID 42384168›Full record

ArticleNaunyn-Schmiedeberg's archives of pharmacology2026

Canonical Wnt pathway mediates catalpol-induced epithelial differentiation of porcine metanephric mesenchymal cells.

Pengcheng Ji, Yuansheng Xie, Guangrui Geng, Wenkai Guo, Jingru Bi, Bing Han, Xu Wang, Bo Fu

Abstract read
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In one paragraph

Article in Naunyn-Schmiedeberg's archives of pharmacology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Pengcheng JiChinese PLA Medical School, Beijing, 100853, China.
Yuansheng XieChinese PLA Medical School, Beijing, 100853, China. xieyuansn@hotmail.com.
Guangrui GengChinese PLA Medical School, Beijing, 100853, China.
Wenkai GuoDepartment of Nephrology, First Medical Center of Chinese PLA General Hospital, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing Key Laboratory of Medical Devices and Integrated Traditional Chinese and Western Drug Development for Severe Kidney Diseases, Beijing Key Laboratory of Digital Intelligent TCM for the Prevention and Treatment of Pan-vascular Diseases, Key Disciplines of National Administration of Traditional Chinese Medicine (zyyzdxk-2023310), Innovation Team and Talents Cultivation Program of National Administration of Traditional Chinese Medicine. (No: ZYYCXTD-D-202402), Beijing, 100853, China.
Jingru BiDepartment of Nephrology, First Medical Center of Chinese PLA General Hospital, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing Key Laboratory of Medical Devices and Integrated Traditional Chinese and Western Drug Development for Severe Kidney Diseases, Beijing Key Laboratory of Digital Intelligent TCM for the Prevention and Treatment of Pan-vascular Diseases, Key Disciplines of National Administration of Traditional Chinese Medicine (zyyzdxk-2023310), Innovation Team and Talents Cultivation Program of National Administration of Traditional Chinese Medicine. (No: ZYYCXTD-D-202402), Beijing, 100853, China.
Bing HanDepartment of Nephrology, Tianjin Union Medical Center, Tianjin, 300121, China.
Xu WangDepartment of Nephrology, First Medical Center of Chinese PLA General Hospital, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing Key Laboratory of Medical Devices and Integrated Traditional Chinese and Western Drug Development for Severe Kidney Diseases, Beijing Key Laboratory of Digital Intelligent TCM for the Prevention and Treatment of Pan-vascular Diseases, Key Disciplines of National Administration of Traditional Chinese Medicine (zyyzdxk-2023310), Innovation Team and Talents Cultivation Program of National Administration of Traditional Chinese Medicine. (No: ZYYCXTD-D-202402), Beijing, 100853, China.
Bo FuDepartment of Nephrology, First Medical Center of Chinese PLA General Hospital, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing Key Laboratory of Medical Devices and Integrated Traditional Chinese and Western Drug Development for Severe Kidney Diseases, Beijing Key Laboratory of Digital Intelligent TCM for the Prevention and Treatment of Pan-vascular Diseases, Key Disciplines of National Administration of Traditional Chinese Medicine (zyyzdxk-2023310), Innovation Team and Talents Cultivation Program of National Administration of Traditional Chinese Medicine. (No: ZYYCXTD-D-202402), Beijing, 100853, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Kidney tissue injury is clinically very common, and how to effectively repair renal damage is a critical scientific issue that urgently requires attention. This study focuses on exploring alternative therapeutic strategies for renal repair. Specifically, it aims to compare the differentiation capabilities of metanephric mesenchymal cells (MMC), bone marrow mesenchymal stem cells (BMSC), and adipose-derived mesenchymal stem cells (AMSC) into epithelial cells, and to investigate the role and mechanism of catalpol, an active component of the traditional Chinese medicine Rehmannia glutinosa, in enhancing the epithelial differentiation of MMC. The epithelial differentiation potential of AMSC, BMSC, and MMC was compared under epithelial induction medium with or without catalpol supplementation. MMC were cultured in epithelial induction medium, and the expression of epithelial marker E-Cadherin, mesenchymal markers α-SMA and renal epithelial-specific marker AQP2 were assessed using Western Blot and immunofluorescence staining to evaluate the epithelial differentiation capacity of MMC and the enhancing effect of catalpol. RNA-seq was performed to identify signaling pathways activated by catalpol in MMC. The canonical Wnt pathway inhibitor MSAB was added to determine whether the pro-epithelial differentiation effect of catalpol was inhibited. AMSC and BMSC exhibited limited epithelial differentiation potential, which was not enhanced by catalpol. Under epithelial induction medium, MMC differentiated into epithelial cells, with increased expression of E-Cadherin and decreased expression of α-SMA. Catalpol further enhanced this effect and promoted the expression of the renal epithelial marker AQP2. RNA-seq revealed that the canonical Wnt pathway was activated in MMC. The pro-epithelial differentiation effect of catalpol was inhibited by MSAB, accompanied by decreased expression of E-Cadherin and AQP2 and increased expression of α-SMA. Catalpol promotes the differentiation of MMC into renal epithelial cells by activating the canonical Wnt pathway.

Indexed as

Cell DifferentiationEpithelial CellsIridoid GlucosidesMesenchymal Stem CellsWnt Signaling PathwayAnimalsCadherinsCells, CulturedSwineCadherinscatalpolIridoid GlucosidesCanonical Wnt pathwayCatalpolEpithelial differentiationMetanephric mesenchymal cells

Identifiers

PMID42384168

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.