Evidence map›Paper›PMID 42383410›Full record

ArticleCurrent protocols2026

A Novel Laboratorial Approach to Evaluate Bacterial Microleakage of Endodontic Sealers.

Emannuel Fonseca Thomé da Silva Monteiro, Alice Abib Ramos Fabri, Marcelo Barbosa Garcia, Sabrina Silva Azevedo, Higor Machado Gonçalves, Ana Clara Miranda Porto Figueiredo, Fabrícia Nogueira Klein, Gabriela Ceccon Chianca, Roberta Barcelos, Helvécio Cardoso Corrêa Póvoa and 2 more

Abstract read
In one paragraph

Article in Current protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Emannuel Fonseca Thomé da Silva MonteiroExperimental and Applied Microbiology Laboratory, Nova Friburgo Institute of Health, Universidade Federal Fluminense (UFF), Nova Friburgo, Rio de Janeiro, Brazil.ORCID https://orcid.org/0009-0007-6168-3019
Alice Abib Ramos FabriExperimental and Applied Microbiology Laboratory, Nova Friburgo Institute of Health, Universidade Federal Fluminense (UFF), Nova Friburgo, Rio de Janeiro, Brazil.ORCID https://orcid.org/0000-0002-8037-9553
Marcelo Barbosa GarciaGraduate Program in Dentistry, Nova Friburgo Institute of Health, UFF, Nova Friburgo, Rio de Janeiro, Brazil.ORCID https://orcid.org/0009-0002-6877-2480
Sabrina Silva AzevedoGraduate Program in Dentistry, Nova Friburgo Institute of Health, UFF, Nova Friburgo, Rio de Janeiro, Brazil.
Higor Machado GonçalvesGraduate Program in Dentistry, Nova Friburgo Institute of Health, UFF, Nova Friburgo, Rio de Janeiro, Brazil.ORCID https://orcid.org/0000-0002-1373-3540
Ana Clara Miranda Porto FigueiredoGraduate Program in Dentistry, Nova Friburgo Institute of Health, UFF, Nova Friburgo, Rio de Janeiro, Brazil.ORCID https://orcid.org/0000-0002-9578-1895
Fabrícia Nogueira KleinExperimental and Applied Microbiology Laboratory, Nova Friburgo Institute of Health, Universidade Federal Fluminense (UFF), Nova Friburgo, Rio de Janeiro, Brazil.ORCID https://orcid.org/0009-0008-6908-5600
Gabriela Ceccon ChiancaExperimental and Applied Microbiology Laboratory, Nova Friburgo Institute of Health, Universidade Federal Fluminense (UFF), Nova Friburgo, Rio de Janeiro, Brazil.ORCID https://orcid.org/0000-0002-9539-0632
Roberta BarcelosDepartment of Specific Formation, Nova Friburgo Institute of Health, UFF, Nova Friburgo, Rio de Janeiro, Brazil.ORCID https://orcid.org/0000-0003-3283-6059
Helvécio Cardoso Corrêa PóvoaExperimental and Applied Microbiology Laboratory, Nova Friburgo Institute of Health, Universidade Federal Fluminense (UFF), Nova Friburgo, Rio de Janeiro, Brazil.ORCID https://orcid.org/0000-0002-2759-2216
Leonardo Santos AntunesGraduate Program in Dentistry, Nova Friburgo Institute of Health, UFF, Nova Friburgo, Rio de Janeiro, Brazil.ORCID https://orcid.org/0000-0002-2115-6958
Natalia Lopes Pontes Póvoa IorioExperimental and Applied Microbiology Laboratory, Nova Friburgo Institute of Health, Universidade Federal Fluminense (UFF), Nova Friburgo, Rio de Janeiro, Brazil.ORCID https://orcid.org/0000-0001-5339-534X

Funding

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
6 · The paper itself

Abstract

To avoid failure of endodontic treatment, it is important to properly seal the root canals after chemomechanical preparation in order to avoid microleakage. Typically, sealing involves the use of gutta-percha and sealing cement, the latter of which adheres to the dentin and fills the spaces between the gutta-percha and the dentin walls. Laboratory studies have been performed to detect microleakage of endodontic sealers based on various criteria, including microbiological ones; however, none guaranteed that the leakage occurred through the root canal space and not through other pathways. This article proposes a new laboratory assay to evaluate bacterial microleakage in endodontic sealers, ensuring visualization of the correct inoculum infiltration pathway. Furthermore, we present the main critical parameters and provide suggestions on how to manage them. These protocols allow the detection of bacterial microleakage in bovine teeth filled with endodontic sealer and inoculated with Enterococcus faecalis. © 2026 The Author(s). Current Protocols published by Wiley Periodicals LLC. Basic Protocol 1: Bovine teeth selection and cleaning Basic Protocol 2: Bovine teeth inclusion Basic Protocol 3: Tooth standardization Basic Protocol 4: Set preparation Basic Protocol 5: Inoculum preparation Basic Protocol 6: Canal inoculation Basic Protocol 7: Set incubation.

Indexed as

Dental LeakageEnterococcus faecalisRoot Canal Filling MaterialsAnimalsCattleRoot Canal Filling Materialsbacteriadental leakageendodonticsendodontic sealermicrobiology

Identifiers

PMID42383410
PMCPMC13320725

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.