ArticleMolecular therapy. Advances2026
CRISPR-Cas9 screening reveals
Article in Molecular therapy. Advances, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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8 authors.
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Abstract
The inherent tendency of human embryonic kidney (HEK) cells to aggregate in suspension culture poses a significant obstacle to the large-scale production of adeno-associated virus (AAV) vectors for gene therapy. To overcome this, we employed a virus-free CRISPR-Cas9 single gene knockout (KO) library in HEK293T cells to systematically identify key genetic regulators of cell aggregation. By serially passaging the KO library and selectively sub-culturing only the suspended (non-aggregated) cells, we enriched for genotypes that reduce aggregation. Next-generation sequencing identified
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