Evidence map›Paper›PMID 42382740›Full record

ArticleFrontiers in immunology2026

Cloning and expression of natively paired antibody fragments from single B cells of immunized rhesus macaques.

Johid Reza Malik, Courtney V Fletcher, Sean N Avedissian

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in immunology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Johid Reza MalikAntiviral Pharmacology Laboratory, College of Pharmacy, University of Nebraska Medical Center, Omaha, NE, United States.
Courtney V FletcherAntiviral Pharmacology Laboratory, College of Pharmacy, University of Nebraska Medical Center, Omaha, NE, United States.
Sean N AvedissianAntiviral Pharmacology Laboratory, College of Pharmacy, University of Nebraska Medical Center, Omaha, NE, United States.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Antigen-binding fragments (Fabs) are emerging biochemical tools for treating and managing several diseases and conditions. Though full-length monoclonal antibodies (mAbs) have great potential, their higher molecular size, time required for generation, and cost remain major limiting factors. Fabs, being smaller and having similar affinity to mAbs for their target, can reach the affected site easily, where penetration of mAb might be hindered. Additionally, with fewer amino acids than mAbs, Fabs can be genetically engineered for the required function. In the present work, we have used a combination of state-of-the-art technologies and classical methods for Fab generation from a single B cell of rhesus macaque previously immunized with factor H binding protein (FHbp) of

Indexed as

Antibodies, BacterialAntigens, BacterialBacterial ProteinsB-LymphocytesImmunoglobulin Fab FragmentsNeisseria meningitidisAnimalsAntibodies, MonoclonalCloning, MolecularImmunizationMacaca mulattaAntibodies, BacterialAntibodies, MonoclonalAntigens, BacterialBacterial Proteinsfactor H-binding protein, Neisseria meningitidisImmunoglobulin Fab FragmentsFABFHBPimmunizationmAbprotein expressionsingle B-cell

Identifiers

PMID42382740
PMCPMC13314517

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.