Targeted amplicon sequencing for enhanced detection of spiked Shiga toxin-producing

Isha Patel, Mark Mammel, Jayanthi Gangiredla, Amit Mukherjee

Abstract read

In one paragraph

Article in Microbiology spectrum, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Isha PatelDivision of Food Safety Genomics, Office of Applied Microbiology and Technology, Human Foods Program, U.S. Food and Drug Administration, Laurel, Maryland, USA.ORCID 0000-0002-0157-4191

Mark MammelDivision of Food Safety Genomics, Office of Applied Microbiology and Technology, Human Foods Program, U.S. Food and Drug Administration, Laurel, Maryland, USA.

Jayanthi GangiredlaDivision of Food Safety Genomics, Office of Applied Microbiology and Technology, Human Foods Program, U.S. Food and Drug Administration, Laurel, Maryland, USA.

Amit MukherjeeDivision of Food Safety Genomics, Office of Applied Microbiology and Technology, Human Foods Program, U.S. Food and Drug Administration, Laurel, Maryland, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The early and accurate detection of low-level pathogenic and indicator organisms in fresh produce is critical for preventing widespread foodborne outbreaks. Contamination of leafy greens with foodborne pathogens, such as Shiga toxin-producing

Indexed as

Food MicrobiologyLactucaShiga-Toxigenic Escherichia coliFoodborne DiseasesFood ContaminationHigh-Throughput Nucleotide SequencingHumansMetagenomicsProof of Concept StudyEscherichia colifoodborne pathogen detectionfood safetymetagenomicsmicrobial surveillancetargeted amplicon sequencing

Identifiers

PMID42379815

DOI10.1128/spectrum.01022-26

PMCPMC13435954

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.