ArticleIn vivo (Athens, Greece)
Time-Dependent Inflammatory Cytokine Responses in a Human Fetal Membrane Dual-Compartment Model.
Article in In vivo (Athens, Greece). The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND/
aimPreterm birth is a leading cause of neonatal morbidity and mortality, with inflammation at the feto-maternal interface playing a central role in its pathogenesis. Human fetal membrane models provide a physiologically relevant platform to investigate inflammatory mechanisms and evaluate targeted interventions. MATERIALS AND
methodsHuman fetal membrane explants from term cesarean deliveries were cultured in a dual-compartment system enabling side-specific stimulation and compartment-resolved analysis. Inflammation was induced by amniotic application of lipopolysaccharide (LPS; 1,000-10,000 ng/ml), and interleukin (IL)-1β and IL-6 concentrations were quantified over 1-48 h. Rytvela (0.1-10 μM), an allosteric IL-1 receptor modulator, was evaluated in a subset of experiments using preceding, simultaneous, and delayed administration relative to LPS exposure.
resultsLPS induced time- and dose-dependent increases in IL-1β and IL-6. IL-6 responses were detectable from 6 h onward, whereas IL-1β increased at later time points (24-48 h). Rytvela alone did not induce cytokine secretion. Modulation of IL-1β responses differed between treatment regimens: while IL-1β levels were increased across all LPS-containing conditions, preceding rytvela was associated with comparatively lower IL-1β levels than simultaneous or delayed administration. In contrast, IL-6 levels were not consistently affected by rytvela across experimental conditions.
conclusionThe dual-compartment fetal membrane model enables time-resolved analysis of inflammatory responses under physiologically relevant conditions. Preceding administration of rytvela was associated with lower IL-1β levels compared to simultaneous or delayed treatment, whereas IL-6 responses were not consistently affected. These findings suggest that timing and pathway-specific regulation may influence inflammatory responses in fetal membranes.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.