Evidence map›Paper›PMID 42377751›Full record

ArticleMolecular biology reports2026

Osthole attenuates cartilage degradation and chondrocyte pyroptosis in knee osteoarthritis and is associated with activation of the PI3K/Akt pathway.

Teng Ma, Yanchen Liang, Jie Yang, Xiaole Wang, Xiangpeng Wang, Wenjing Qu, Yongkui Zhang, Wenpeng Xie

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Article in Molecular biology reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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8 authors.

Teng Ma *First Clinical Medical College, Shandong University of Traditional Chinese Medicine, Jinan, China.
Yanchen Liang *Department of Orthopedics, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, China.
Jie YangDepartment of Neurology, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, China.
Xiaole WangDepartment of Orthopedics, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, China.
Xiangpeng WangFirst Clinical Medical College, Shandong University of Traditional Chinese Medicine, Jinan, China.
Wenjing QuFirst Clinical Medical College, Shandong University of Traditional Chinese Medicine, Jinan, China.
Yongkui ZhangDepartment of Orthopedics, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, China. 71000356@sdutcm.edu.cn.
Wenpeng XieDepartment of Orthopedics, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, China. 71002083@sdutcm.edu.cn.

Funding

Joint project between the Science and Technology Department of the National Administration of Traditional Chinese Medicine and the Health Commission of Shandong Province GZY-KJS-SD-2024-087Medical and Health Science and Technology Development Project of Shandong Province 202304071516National Natural Science Foundation of China 82104897Natural Science Foundation of Shandong Province ZR2022QH208Traditional Chinese Medicine science and technology projects of Shandong Province Q-2022063
6 · The paper itself

Abstract

objectiveTo verify the protective effect of osthole on knee osteoarthritis (KOA) and explore its mechanism of action.

methodsRats receiving the modified Hulth method and IL-1β-induced chondrocytes were used to construct in vivo and in vitro KOA models, respectively, and were subsequently treated by osthole. Hematoxylin-eosin and safranin O/fast green staining assays were used to assess the histological effects of osthole administered by oral gavage on the knee joint cartilage of KOA model rats. Western blotting, qRT-PCR, and immunofluorescence and immunohistochemical staining analyses were used to examine the expression of factors related to cartilage degeneration and chondrocyte pyroptosis in vitro and in vivo. The potential molecular targets of osthole action on KOA and their relevant pathways were predicted and verified.

resultsOsthole alleviated cartilage injury and lowered pathology scores in KOA rats. Osthole increased the levels of collagen Ⅱ but decreased those of ADAMTS-5, MMP3, and MMP13 in rat chondrocytes. Chondrocyte pyroptosis was alleviated by osthole, as evidenced by decreased levels of NLRP3, caspase-1, ASC, GSDMD, IL-1β, and IL-18 in rat chondrocytes and serum. Osthole prevented LDH release in rat chondrocytes induced by IL-1β. Bioinformatics analysis showed that osthole targets the PI3K/Akt pathway. PI3K inhibitor LY294002 treatment reversed the effects of osthole on chondrocyte degeneration and pyroptosis.

conclusionOsthole alleviated cartilage degradation and chondrocyte pyroptosis in KOA, and these effects were associated with activation of the PI3K/Akt signaling pathway.

Indexed as

ChondrocytesCoumarinsOsteoarthritis, KneePyroptosisAnimalsCartilage, ArticularChromonesDisease Models, AnimalInterleukin-1betaMaleMorpholinesPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktRatsRats, Sprague-DawleySignal TransductionChromonesCoumarinsInterleukin-1betaMorpholinesostholPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktChondrocyteKnee osteoarthritisMolecular dockingOstholePI3K/Akt signaling pathwayPyroptosis

Identifiers

PMID42377751

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