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ArticleMolecular biology reports2026

Genome analysis and introgression of multiple disease resistance into pre-breeding lines derived from greengram × ricebean crosses.

P Venkatesh, K Anandhi, L Karthiba, P Renukadevi, S Vellaikumar, T Nivethitha, P Jayamani

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Article in Molecular biology reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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7 authors.

P VenkateshCentre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore, 641 003, Tamil Nadu, India.
K AnandhiCentre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore, 641 003, Tamil Nadu, India.
L KarthibaDepartment of Plant Pathology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore, 641 003, Tamil Nadu, India.
P RenukadeviDepartment of Plant Pathology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore, 641 003, Tamil Nadu, India.
S VellaikumarDepartment of Plant Molecular Biology & Bioinformatics, Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore, 641 003, Tamil Nadu, India.
T NivethithaCentre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore, 641 003, Tamil Nadu, India.
P JayamaniCentre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore, 641 003, Tamil Nadu, India. jayamani.p@tnau.ac.in.

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6 · The paper itself

Abstract

backgroundGreengram (Vigna radiata) productivity is constrained by major pests and diseases, and introgression of ricebean (Vigna umbellata) derived genomic segments conferring disease resistance through interspecific hybridization offers an effective strategy to broaden the genetic base and enhance resistance to biotic stresses. METHODS AND

resultsIn the present study, three advanced pre-breeding lines (COGRISC 1, COGRISC 2 and COGRISC 3) developed through interspecific crosses between greengram (CO 6 and CO 7) and ricebean (RBL 35 and LRB 576), were evaluated. These lines exhibited ricebean-like qualitative traits, including hypogeal germination and purple hypocotyl colour, while biometrical traits showed intermediate expression between the parents. The pre-breeding lines exhibited partial fertility, ranged from 37.50 to 78.50%. To confirm ricebean genome introgression, 155 adzuki bean-derived SSR markers were screened for parental polymorphism, of which 124 (80%) were polymorphic and used for genotyping. Ricebean-specific marker alleles predominated in the pre-breeding lines, with 71 (57.30%) markers detected in COGRISC 1 and COGRISC 2 and 65 (52.40%) markers in COGRISC 3. Graphical genotype analysis revealed a mosaic pattern of introgression across all linkage groups with predominance of ricebean genomic segments. Cluster analysis grouped ricebean parents and pre-breeding lines together. All lines exhibited disease-free reactions against Mungbean Yellow Mosaic Virus (MYMV), powdery mildew, and leaf crinkle disease under artificial screening, and to cercospora leaf spot under field conditions.

conclusionsThe results demonstrate that ricebean-derived genomic segments confer multiple disease resistance, highlighting the potential of these lines as donor material for greengram improvement.

Indexed as

Disease ResistanceVignaChromosome MappingCrosses, GeneticGenetic IntrogressionGenome, PlantGenotypeHybridization, GeneticMicrosatellite RepeatsPhenotypePlant BreedingPlant DiseasesDisease resistanceGreengramInterspecific crossPre-breeding linesRicebeanSSR markers

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.