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ArticlePlant cell reports2026

Identification of the R2R3-MYB transcription factor family in goji and LbaR2R3-MYB3 mediated wax biosynthesis enhancing powdery mildew resistance in tobacco.

Xingbin Wang, Jie Li, Sitian Li, Xiao Zhang, Jing Wang, Tong Hou, Jing He

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Article in Plant cell reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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7 authors.

Xingbin Wang *College of Forestry, Gansu Agricultural University, Lanzhou, 730070, China.
Jie Li *College of Forestry, Gansu Agricultural University, Lanzhou, 730070, China. lj81658@gsau.edu.cn.
Sitian LiCollege of Horticulture, Gansu Agricultural University, Lanzhou, 730070, China.
Xiao ZhangCollege of Forestry, Gansu Agricultural University, Lanzhou, 730070, China.
Jing WangCollege of Forestry, Gansu Agricultural University, Lanzhou, 730070, China.
Tong HouCollege of Forestry, Gansu Agricultural University, Lanzhou, 730070, China.
Jing HeCollege of Forestry, Gansu Agricultural University, Lanzhou, 730070, China.

Funding

Gansu Province Natural Science Foundation 24JRRA650Gansu Provincial Education Science and Technology Innovation Project 2026A-063National Natural Science Foundation of China 32060341
6 · The paper itself

Abstract

key messageThe transcription factor LbaR2R3-MYB3 of goji promotes the biosynthesis of wax components including alkanes, alcohols, fatty acids, and ketones, increases total leaf cuticular wax load, and consequently enhances tobacco resistance to powdery mildew. Goji (Lycium barbarum) is one of the important economic forest species in northwest China. Powdery mildew is a common disease of goji plants that significantly reduces yield and quality, causing substantial economic losses for growers. Cuticular wax serves as the first physical barrier protecting plants against both biotic and abiotic stresses. Members of the R2R3-MYB transcription factor family in goji have not been previously reported, and the relationship between the key member LbaR2R3-MYB3 and cuticular wax biosynthesis and powdery mildew resistance remains unclear. Therefore, this study employed "Ningqi I" goji as the experimental material and identified 75 R2R3-MYB transcription factor family members distributed across 11 chromosomes in the genome. The goji LbaR2R3-MYB proteins mainly localized in the nucleus, mitochondria, cytoplasm, and chloroplasts. Ten conserved motifs were identified in LbaR2R3-MYB3 proteins, with Motif1, Motif2, and Motif3 being the most widely distributed. The promoters of these family members contain multiple cis-acting elements responsive to hormones and abiotic stress. Members of the LbaR2R3-MYB transcription factor family exhibited evolutionary instability. The LbaR2R3-MYB3 gene was cloned from goji leaves yielding a 756 bp fragment. Heterologous expression vectors were successfully constructed, and transgenic tobacco lines overexpressing LbaR2R3-MYB3 (LbaR2R3-MYB3-OE) were obtained. Compared with wild-type tobacco, the cuticular wax load in transgenic tobacco leaves was significantly increased by 1.82-fold, with significant increases in alkanes, alcohols, fatty acids, and ketones. The transgenic tobacco lines showed a 38.2% reduction in powdery mildew incidence and a 59.8% decrease in disease index compared to WT, indicating significantly enhanced resistance to powdery mildew. These results demonstrate that the transcription factor LbaR2R3-MYB3 promotes the biosynthesis of wax components including alkanes, alcohols, fatty acids, and ketones, increases total leaf cuticular wax load, and consequently enhances tobacco resistance to powdery mildew.

Indexed as

AscomycotaDisease ResistanceNicotianaPlant DiseasesPlant ProteinsTranscription FactorsWaxesAmino Acid SequenceGene Expression Regulation, PlantMultigene FamilyPhylogenyPlant LeavesPlants, Genetically ModifiedPlant ProteinsTranscription FactorsWaxesCuticular waxGoji berryLbaR2R3-MYB3Powdery mildewWax component

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