Evidence map›Paper›PMID 42377026›Full record

ArticleTransboundary and emerging diseases2026

Development of a High-Resolution Melting (HRM)-Based Multiplex Real-Time PCR Assay of PEDV, TGEV, PoRV, PDCoV, PRV, and PRRSV.

Fangshan Wang, Jing Ren, Yawen Wang, Chen Yuan, Tairan Sun, Jinhai Huang, Qinye Song

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Transboundary and emerging diseases, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Fangshan WangCollege of Veterinary Medicine, Hebei Agricultural University, Baoding, 071000, Hebei, China, hebau.edu.cn.
Jing RenCollege of Veterinary Medicine, Hebei Agricultural University, Baoding, 071000, Hebei, China, hebau.edu.cn.
Yawen WangCollege of Veterinary Medicine, Hebei Agricultural University, Baoding, 071000, Hebei, China, hebau.edu.cn.
Chen YuanCollege of Veterinary Medicine, Hebei Agricultural University, Baoding, 071000, Hebei, China, hebau.edu.cn.
Tairan SunBaoding Animal Disease Control and Prevention Center, Baoding, 071000, Hebei, China.
Jinhai HuangSchool of Life Sciences, Faculty of Medicine, Tianjin University, Tianjin, 300072, China, tju.edu.cn.
Qinye SongCollege of Veterinary Medicine, Hebei Agricultural University, Baoding, 071000, Hebei, China, hebau.edu.cn.ORCID https://orcid.org/0000-0002-2899-8517

Funding

Enterprise-University-Research Cooperation Project for Hebei-based Universities of Shijiazhuang Municipal Government 241500417AJoint Special Project of Natural Science Foundation of Beijing-Tianjin-Hebei Region 25JJJJC0018
6 · The paper itself

Abstract

Six key viral pathogens-porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV), porcine rotavirus (PoRV), porcine deltacoronavirus (PDCoV), pseudorabies virus (PRV), and porcine reproductive and respiratory syndrome virus (PRRSV)-inflict substantial economic losses on the global swine industry. Their frequent cocirculation and the resulting mixed infections severely complicate rapid and accurate diagnosis. To overcome this challenge, we developed a multiplex real-time PCR assay integrated with high-resolution melting (HRM) analysis for their simultaneous detection and differentiation. Specific primer sets were designed and optimized to target conserved regions of the PEDV-M, TGEV-N, PoRV-VP6, PDCoV-M, PRV-gE, and PRRSV-ORF5 genes. Reaction conditions and HRM parameters were refined, ensuring specific amplification and distinct melting profiles for all six viruses. The assay exhibited excellent specificity, showing no cross-reactivity with other common porcine pathogens. Limits of detection (LODs) were established at 10 copies/μL for five viruses (PEDV, TGEV, PoRV, PDCoV, and PRRSV) and 100 copies/μL for PRV. Amplification efficiency ranged from 90% to 102% (R

Indexed as

Multiplex Polymerase Chain ReactionReal-Time Polymerase Chain ReactionSwine DiseasesAnimalsDeltacoronavirusHerpesvirus 1, SuidPorcine epidemic diarrhea virusPorcine respiratory and reproductive syndrome virusRotavirusSensitivity and SpecificitySwineTransmissible gastroenteritis virushigh-resolution melting (HRM)major porcine viral pathogensmultiplex real-time PCRsimultaneous and differential detection

Identifiers

PMID42377026
PMCPMC13317146

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.