Evidence map›Paper›PMID 42376734›Full record

ArticleChembiochem : a European journal of chemical biology2026

Mutasynthesis and Antibiotic Activity of Mupirocin Analogues.

Sarah M Husain, Luoyi Wang, Li-Chen Han, James I Bowen, Zhongshu Song, Felix de Courcy-Ireland, Ashley J Winter, Thomas J Simpson, Paul R Race, James Spencer and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Chembiochem : a European journal of chemical biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Mutasynthesis and Antibiotic Activity of Mupirocin Analogues.Chembiochem : a European journal of chemical biology · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Sarah M HusainSchool of Chemistry, Cantock's Close, University of Bristol, Bristol, UK.
Luoyi WangSchool of Chemistry, Cantock's Close, University of Bristol, Bristol, UK.ORCID https://orcid.org/0000-0003-2032-7206
Li-Chen HanSchool of Chemistry, Cantock's Close, University of Bristol, Bristol, UK.
James I BowenSchool of Chemistry, Cantock's Close, University of Bristol, Bristol, UK.ORCID https://orcid.org/0000-0002-1596-4174
Zhongshu SongSchool of Chemistry, Cantock's Close, University of Bristol, Bristol, UK.
Felix de Courcy-IrelandSchool of Chemistry, Cantock's Close, University of Bristol, Bristol, UK.ORCID https://orcid.org/0000-0003-1878-8732
Ashley J WinterSchool of Chemistry, Cantock's Close, University of Bristol, Bristol, UK.ORCID https://orcid.org/0000-0003-3684-9383
Thomas J SimpsonSchool of Chemistry, Cantock's Close, University of Bristol, Bristol, UK.ORCID https://orcid.org/0000-0003-0777-1935
Paul R RaceSchool of Natural and Environmental Sciences, Newcastle University, Newcastle upon Tyne, UK.ORCID https://orcid.org/0000-0003-0184-5630
James SpencerSchool of Cellular and Molecular Medicine, University of Bristol, Bristol, UK.ORCID https://orcid.org/0000-0002-4602-0571
Matthew P CrumpSchool of Chemistry, Cantock's Close, University of Bristol, Bristol, UK.ORCID https://orcid.org/0000-0002-7868-5818
Christine L WillisSchool of Chemistry, Cantock's Close, University of Bristol, Bristol, UK.ORCID https://orcid.org/0000-0002-3919-3642

Funding

Biotechnology and Biological Sciences Research Council BB/L01386X/1Biotechnology and Biological Sciences Research Council BB/R007853/1Biotechnology and Biological Sciences Research Council BB/T001968/1Biotechnology and Biological Sciences Research Council BB/W008823/1Engineering and Physical Sciences Research Council EP/S024107/1
6 · The paper itself

Abstract

With growing understanding of complex biosynthetic pathways to natural products, mutasynthesis, which combines metabolic engineering with chemical synthesis, is becoming an increasingly important tool to produce novel compounds. Mupirocin, isolated from Pseudomonas fluorescens, is a mixture of pseudomonic acids (PAs) that exhibit antibiotic activity against Gram-positive bacteria including methicillin-resistant Staphylococcus aureus. We have developed a flexible approach, based on mutasynthesis, for the preparation of a library of novel PA analogues. The antimicrobial activities of the natural products and synthetic analogues were evaluated against Bacillus subtilis and four Staphylococcus aureus strains. Interestingly, one of the analogues retained antimicrobial activity in all the assays but lacked structural features that render PA-A unstable, that is, the 10,11-epoxide (replaced by an alkene) and ester linkage (replaced by a ketone). In addition, mutasynthesis allowed the preparation of an analogue of a key biosynthetic intermediate (desepoxy-PA-B) in which the C

Indexed as

Anti-Bacterial AgentsMetabolic EngineeringMupirocinBacillus subtilisMicrobial Sensitivity TestsPseudomonas fluorescensStaphylococcus aureusAnti-Bacterial AgentsMupirocinantimicrobialBacillus subtilisbiosynthesismetabolic engineeringmupirocinpolyketidePseudomonas fluorescenssynthesis

Identifiers

PMID42376734
PMCPMC13316724

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.