ArticleOpen veterinary journal2026
Comparative
Article in Open veterinary journal, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Authors and funding
3 authors.
Funding
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Abstract
Background: Prostate cancer is one of the most common cancers in men. Current treatments include surgery, radiotherapy, and chemotherapy. These options improve survival but often cause serious side effects. Therefore, researchers are looking for natural products that may provide safer anticancer effects. Broccoli ( Aim: This study aimed to compare the cytotoxic effects of aqueous extracts from broccoli and mushroom. The cytotoxic activity of aqueous extracts of broccoli and mushroom was evaluated on human prostate cancer cells and normal human foreskin fibroblast-1 (HFF-1) cells. This study used the total aqueous extract of broccoli without separating specific phytochemicals. No quantification of a specific ingredient was included because the focus was on the overall cytotoxic effect. Methods: Extracts were prepared by drying, grinding, and dissolving the plant material in distilled water. They were then tested at concentrations between 25 and 800 μg/ml. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to measure cell viability after 24 hours of treatment. Normal HFF-1 levels were also tested for safety. Morphological changes were observed using SEM. Results: Both extracts reduced cancer cell viability in a dose-dependent manner. At 800 μg/ml, broccoli extract reduced viability to 56.75%, whereas mushroom extract reduced viability to 41.09%. The IC Conclusion: Mushroom extract was more effective than broccoli extract in reducing prostate cancer cell viability.
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Registered trials
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