Evidence map›Paper›PMID 42376099›Full record

ArticleOpen veterinary journal2026

ELISA-based assessment of cholinesterase levels in different animal groups: Insights into neurological health.

Reem M Mousa, Azhar Adil Mohammad, Ayman Albanna

Abstract read
In one paragraph

Article in Open veterinary journal, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Reem M MousaBasic Nursing Sciences Branch, Nursing College, Ninevah University, Mosul, Iraq.
Azhar Adil MohammadBasic Nursing Sciences Branch, Nursing College, Ninevah University, Mosul, Iraq.
Ayman AlbannaDepartment of Environmental Science, College of Environmental Sciences, University of Mosul, Mosul, Iraq.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Neurological health is a relevant area of animal public health, as perturbations in the activity of neural enzymes have a tendency to mirror animal welfare and environmental stress that will ultimately affect human populations. Aim: The aim was to measure serum cholinesterase (ChE) activity in different animal species using a 96-well Enzyme-Linked Immunosorbent Assay (ELISA) assay, with an emphasis on its application as a biomarker of animal welfare within the frame of public health. Methods: A linear calibration curve (y = 0.0062x + 0.2011, Results: The findings demonstrated distinct interspecies variations, revealing the highest mean enzyme activity in chickens (6.99 U/l), followed by cats and fish, and the lowest in sheep, goats, and dogs (1.6-1.7 U/l). The results show that the activity of ChE is directly related to the physiological status of animals and may be a highly sensitive biochemical parameter for measuring animal welfare and neurophysiological homeostasis. There could be reduced activity of ChE could be indicative of exposure to neurotoxic chemicals, stress, or starvation, while consistent activity would indicate health and environmental stability. Conclusion: From the public health point of view, the study highlights the importance of monitoring ChE activity as being integral to welfare monitoring and environmental watch programs. The ELISA-based assay was shown to be a high-throughput, robust method for determining neurological and welfare status in animals, reaffirming its usefulness under the One Health umbrella that links animal and human well-being.

Indexed as

CholinesterasesAnimalsAnimal WelfareBiomarkersCatsDogsEnzyme-Linked Immunosorbent AssaySpecies SpecificityBiomarkersCholinesterasesAnimal public healthCholinesterase levelsELISA

Identifiers

PMID42376099
PMCPMC13314357

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LicenceCC BY-NC
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.