ArticleOpen veterinary journal2026
The effect of toll-like receptor 7/8 activation by ligand (R848) on morphometry, proportion of X-Y chromosome-bearing sperm, and sexed sperm quality of bucks.
Article in Open veterinary journal, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Background: Sperm separation is a reproductive biotechnology that separates X- and Y-chromosome-bearing sperm to predetermine offspring sex, offering major benefits for livestock breeding programs. The discovery of an easy, inexpensive, and effective method will be very useful as an alternative to flow cytometry. The swim-up method is a method that meets these criteria. Combining the swim-up method with the addition of a substance to separate X- and Y-chromosome-bearing sperm is very helpful in developing more efficient methods. The activation of TLR 7/8 using the ligand R848 is related to sperm morphometry, the proportion of X and Y chromosome-bearing sperm, and sexed sperm quality. Aim: This study aimed to determine the effect of activating TLR 7/8 using the ligand R848 on sperm morphometry, the proportion of X- and Y-chromosome-bearing sperm, and sexed sperm quality in bucks. Methods: Fresh semen samples were collected from 3-year-old bucks using an artificial vagina. A completely random design was used with four treatment levels of ligand (R848) and seven replications. The level treatment of ligand (R848) consisted of T Results: Sperm length ranged from 7.02 to 9.99 µm and 8.54 µm on average. Sperm width ranges from 3.08 to 6.01 µm, with an average of 4.61 µm. The sperm head area ranges from 28.02 to 34.14 µm Conclusion: Ligand (R848)-mediated activation of Toll-like receptors 7/8 selectively suppresses motility of X-chromosome-bearing sperm in buck, enabling effective enrichment of X-sperm via simple swim-up separation. Morphometric analysis, motility assessment, and immunofluorescence analysis confirmed that the bottom fraction was significantly enriched in functionally impaired X-sperm.
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