Evidence map›Paper›PMID 42375517›Full record

ArticleiScience2026

RT-qPCR detection of dsRNA pesticides: Optimization, limitations, and formulation effects.

Venetia Koidou, Evangelia Tamvakologou, Minlee Kim, Dimitrios G Karpouzas, Kalliope Κ Papadopoulou, Athanasios Dalakouras

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in iScience, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Venetia KoidouELGO-DIMITRA, Institute of Industrial and Forage Crops, Theofrastou 1, 41335 Larissa, Greece.
Evangelia TamvakologouUniversity of Thessaly, Department of Biochemistry and Biotechnology, Laboratory of Plant and Environmental Biotechnology, Biopolis Campus 415 00, Larissa, Greece.
Minlee KimGenolution Inc., 63, Magokjungang 8-ro 3-gil, Gangseo-gu, Seoul, Korea.
Dimitrios G KarpouzasUniversity of Thessaly, Department of Biochemistry and Biotechnology, Laboratory of Plant and Environmental Biotechnology, Biopolis Campus 415 00, Larissa, Greece.
Kalliope Κ PapadopoulouUniversity of Thessaly, Department of Biochemistry and Biotechnology, Laboratory of Plant and Environmental Biotechnology, Biopolis Campus 415 00, Larissa, Greece.
Athanasios DalakourasELGO-DIMITRA, Institute of Industrial and Forage Crops, Theofrastou 1, 41335 Larissa, Greece.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Double-stranded RNA (dsRNA)-based pesticides are emerging as highly specific and environmentally sustainable crop protection agents. However, their environmental fate remains poorly characterized due to the lack of standardized analytical methods. Here, we developed a quantitative reverse-transcription PCR (RT-qPCR) workflow for quantifying naked and chitosan-formulated dsRNA in laboratory preparations and agricultural soils. Using a 297-bp dsRNA targeting

Indexed as

Analytical chemistryEnvironmental monitoringSoil science

Identifiers

PMID42375517
PMCPMC13312021

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.