ArticleOpen veterinary journal2026
Molecular detection and phylogenetic characterization of
Article in Open veterinary journal, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Background: Leptospirosis is a major worldwide health concern caused by pathogenic spirochetes from the genus Aim: This study aimed to find Methods: Eighty blood and urine samples were collected from sheltered dogs under general anesthesia between May and August 2025. The samples were then subjected to DNA extraction and conventional polymerase chain reaction that targeted the conserved secY gene using phylogenetic analysis and sequencing. Results: Leptospirosis DNA was found in 23 samples (28.75%), including 11 urine samples (13.75%) and 12 blood samples (15%). Male dogs aged 2-3 years had the highest incidence of infection (23.1%), whereas female dogs had the highest rate (23.5%). Conclusion: These results highlight the critical need for comprehensive surveillance programs, enhanced biosecurity procedures, and vaccination plans in shelter settings throughout the Kurdistan Region.
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