Evidence map›Paper›PMID 42375453›Full record

ArticleOpen veterinary journal2026

Development and evaluation of a duplex recombinase polymerase amplification assay for rapid detection of

Jingjing Li, Xujie Zhao, Qianlei Zhu, Jiahui Fu, Mingzhu Zhou, Yilin Bai, Xiaobing Wei, Meinan Chang, Yueyu Bai, Jianhe Hu and 2 more

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In one paragraph

Article in Open veterinary journal, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Jingjing LiCollege of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang, China.
Xujie ZhaoCollege of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang, China.
Qianlei ZhuCollege of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang, China.
Jiahui FuCollege of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang, China.
Mingzhu ZhouCollege of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang, China.
Yilin BaiLaboratory of Indigenous Cattle Germplasm Innovation, School of Agricultural Sciences, Zhengzhou University, Zhengzhou, China.
Xiaobing WeiCollege of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang, China.
Meinan ChangCollege of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang, China.
Yueyu BaiAnimal Health Supervision of Henan Province, Bureau of Animal Husbandry of Henan Province, Zhengzhou, China.
Jianhe HuCollege of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang, China.
Ke DingCollege of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang, China.
Xiaojing XiaCollege of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Bovine mastitis caused by Aim: This study aimed to develop and validate a duplex recombinase polymerase amplification (RPA) assay for the simultaneous, rapid, and sensitive detection of GBS and KP in bovine mastitis. Methods: Conserved cfb (GBS) and rcsA (KP) genes were targeted, and specific primer pairs were designed for duplex RPA assay development. The reaction conditions, including amplification temperature, time, and primer ratios, were optimized. Analytical sensitivity was assessed using serial dilutions of genomic DNA, and specificity was evaluated against 6 non-target bacterial species. Clinical applicability was determined by testing 35 milk samples from cows with mastitis using duplex RPA and conventional polymerase chain reaction (PCR). Results: The optimized duplex RPA assay was completed within 30 minutes at 39°C with balanced primer concentrations, enabling the simultaneous detection of GBS and KP. The assay exhibited high specificity with no cross-reactions. The detection limit was 10 Conclusion: Duplex RPA enabled the rapid, specific, and sensitive detection of GBS and KP in bovine mastitis, with superior analytical sensitivity compared with PCR.

Indexed as

Klebsiella InfectionsKlebsiella pneumoniaeMastitis, BovineNucleic Acid Amplification TechniquesPolymerase Chain ReactionStreptococcal InfectionsStreptococcus agalactiaeAnimalsCattleFemaleMilkRapid Diagnostic TestsSensitivity and SpecificityBovine mastitisDuplex detectionKlebsiella pneumoniaeRPAStreptococcus agalactiae

Identifiers

PMID42375453
PMCPMC13313798

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.