Evidence map›Paper›PMID 42375452›Full record

ArticleOpen veterinary journal2026

Development of a multiplex polymerase chain reaction assay for the detection of Piroplasma (

Jiravich Methawiroon, Piangjai Chalermwong, Ornarin Boonjint, Piangdow Chiawvitkan, Sakulchit Wichianchot, Chanapath Thabthimsri, Preeyanuch Thongpoo, Ketsarin Kamyingkird, Sathaporn Jittapalapong, Eukote Suwan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Open veterinary journal, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Jiravich MethawiroonDepartment of Veterinary Technology, Faculty of Veterinary Technology, Kasetsart University, Bangkok, Thailand.
Piangjai ChalermwongDepartment of Veterinary Technology, Faculty of Veterinary Technology, Kasetsart University, Bangkok, Thailand.
Ornarin BoonjintDepartment of Veterinary Technology, Faculty of Veterinary Technology, Kasetsart University, Bangkok, Thailand.
Piangdow ChiawvitkanDepartment of Veterinary Technology, Faculty of Veterinary Technology, Kasetsart University, Bangkok, Thailand.
Sakulchit WichianchotDepartment of Veterinary Technology, Faculty of Veterinary Technology, Kasetsart University, Bangkok, Thailand.
Chanapath ThabthimsriFaculty of Veterinary Technology, Kasetsart University, Bangkok, Thailand.
Preeyanuch ThongpooProgram of Science (Biology), Faculty of Science and Technology, Phuket Rajabhat University, Phuket, Thailand.
Ketsarin KamyingkirdDepartment of Parasitology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, Thailand.
Sathaporn JittapalapongFaculty of Veterinary Technology, Kasetsart University, Bangkok, Thailand.
Eukote SuwanDepartment of Veterinary Technology, Faculty of Veterinary Technology, Kasetsart University, Bangkok, Thailand.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Blood parasites cause serious drawbacks in the livestock industry. The detection methods rely on microscopic examination and polymerase chain reaction (PCR), which requires an expert person, labor, time, and cost. Aim: In this study, a multiplex PCR has been developed for hemoparasites consisting of Piroplasma ( Methods: Blood parasite infections in cattle were investigated using three different methods: microscopic examination, single PCR, and multiplex PCR. The Results: Multiplex PCR assay revealed a limit of detection of 0.01-10 pg of parasite DNA. According to the evaluation of 60 bovine blood samples, blood smear, single PCR, and multiplex PCR revealed 50.0%, 18.3%, and 26.7% of single infections, and 40.0%, 81.7%, and 50.0% of co-infections, respectively. The comparative analysis between multiplex PCR with microscopic examination and single PCR revealed that triple infection, Conclusion: The multiplex PCR assay developed in this study may be helpful for improved hemoparasite prevention and control when combined with farmer education, proper hygiene practices, and effective environmental management.

Indexed as

AnaplasmosisBabesiosisCattle DiseasesMultiplex Polymerase Chain ReactionTheileriasisTrypanosomiasisAnaplasmaAnimalsBabesiaCattleSensitivity and SpecificityTheileriaTrypanosomaHemoparasitesMultiplex PCRPiroplasmaTrypanosoma evansi.

Identifiers

PMID42375452
PMCPMC13314195

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.