Evidence map›Paper›PMID 42375102›Full record

ArticleAnimal genetics2026

Transcriptomic Analysis Reveals Multilayer Regulation Underlying Development and Pregnancy in the Malayan Pangolin.

Xianghui Liang, Miaomiao Jia, Zhiguang Wang, YuShan Ding, Fanghui Hou, Chengwei Xiang, Wanhe Huang, He Wang, Xuelin Xu, Yingjin Mao and 5 more

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In one paragraph

Article in Animal genetics, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

15 authors.

Xianghui LiangCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
Miaomiao JiaGuangxi Forestry Research Institute, Nanning, China.
Zhiguang WangGuangdong Wildlife Monitoring and Rescue Center, Guangzhou, China.
YuShan DingGuangzhou Zoo & Guangzhou Wildlife Research Center, Guangzhou, China.
Fanghui HouGuangdong Wildlife Monitoring and Rescue Center, Guangzhou, China.
Chengwei XiangGuangdong Wildlife Monitoring and Rescue Center, Guangzhou, China.
Wanhe HuangGuangdong Wildlife Monitoring and Rescue Center, Guangzhou, China.
He WangGuangdong Wildlife Monitoring and Rescue Center, Guangzhou, China.
Xuelin XuGuangdong Wildlife Monitoring and Rescue Center, Guangzhou, China.
Yingjin MaoGuangdong Wildlife Monitoring and Rescue Center, Guangzhou, China.
Luman ChenGuangdong Wildlife Monitoring and Rescue Center, Guangzhou, China.
Guangda YangGuangdong Wildlife Monitoring and Rescue Center, Guangzhou, China.
Yongyi ShenCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, China.ORCID https://orcid.org/0000-0001-7660-5522
Wu ChenGuangzhou Zoo & Guangzhou Wildlife Research Center, Guangzhou, China.
Xuejuan ShenSchool of Life and Health Sciences, Hainan Province Key Laboratory of One Health, Hainan University, Haikou, Hainan, China.

Funding

Forestry Science and Technology Innovation project in Guangdong Province 2025KJCX003Guangxi Key Laboratory of Special Non-wood Forests Cultivation and Utilization Fund JA-25-04-02Natural Science Foundation of Guangxi - Young Scientists Fund 2024GXNSFBA010369
6 · The paper itself

Abstract

The critically endangered Malayan pangolin (Manis javanica) exhibits distinctive physiological adaptations; nevertheless, its molecular mechanisms underlying development and reproduction remain inadequately characterized, thereby impeding effective conservation initiatives. In this study, we conducted a comprehensive transcriptomic analysis of six tissues (intestine, kidney, liver, lung, muscle, and spleen) derived from adult, fetal, and pregnant individuals. The fetal stage demonstrated the greatest abundance of stage-specific genes, which were predominantly enriched in developmental pathways such as epithelial organization and transmembrane signaling. Differential gene expression (DGE) analysis revealed that genes upregulated in adults were primarily associated with metabolic and immune functions, whereas those upregulated in fetuses were linked to developmental processes and cell cycle regulation. Co-expression network analysis identified critical hub genes, including GLDC in adults and NCAM1 in fetuses, that are implicated in stage-specific regulatory mechanisms. Additionally, extensive differential alternative splicing (DAS) events were observed, with exon skipping (SE) representing the most frequent splicing pattern. Integrative analyses suggested that DGE and DAS largely constitute independent regulatory layers; however, a subset of hub genes, such as OBSL1 and TNIP1, exhibited concurrent differential expression and splicing, indicating their potential central roles in coordinating developmental processes. This study presents a detailed transcriptomic atlas of Malayan pangolin development and pregnancy, providing candidate biomarker genes for evaluating reproductive health and developmental status, thereby informing future conservation strategies.

Indexed as

Gene Expression Regulation, DevelopmentalPangolinsTranscriptomeAlternative SplicingAnimalsFemaleGene Expression ProfilingPregnancyalternative splicingco‐expression networkMalayan pangolinstage‐specific gene expressiontranscriptome

Identifiers

PMID42375102
PMCPMC13480346

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