ArticleJournal of the science of food and agriculture2026
Engineering and application of a recombinant Aspergillus niger CECT 2907 tannase to enhance the antioxidant activity of black tea.
Article in Journal of the science of food and agriculture, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
backgroundTannases (tannin acyl hydrolases, EC 3.1.1.20) catalyze the hydrolysis of galloylated polyphenols, releasing gallic acid and other bioactive compounds with antioxidant properties. These enzymes have a wide range of applications in the food and beverage industry, particularly for improving tea quality. In this study, a tannase gene from Aspergillus niger CECT 2907 was cloned and heterologously expressed in Komagataella phaffii. Two recombinant variants were generated: a double-chain enzyme (AnTanDC) and a single-chain variant (AnTanSC) obtained by mutating two Kex2 protease cleavage sites. Both enzymes were biochemically characterized, and the potential of AnTanSC to enhance the antioxidant properties of black tea was evaluated.
resultsBoth recombinant tannases were efficiently secreted and produced in 5 L bioreactors, reaching activities of 4238 ± 16 U L
conclusionThe engineered single-chain tannase AnTanSC combined improved thermal stability with effective enhancement of the antioxidant properties of black tea, supporting its potential application as a biocatalyst for the processing of tea beverages and other polyphenol-rich foods. © 2026 Society of Chemical Industry.
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