Evidence map›Paper›PMID 42373639›Full record

ArticleNature communications2026

Gas-phase unfolding assay rapidly predicts structure-function relationships in engineered antibodies with tuned flexibilities.

Rosendo C Villafuerte-Vega, Michael R Armbruster, Hayden Fisher, Isabel G Elliott, H T Claude Chan, Tatyana Inzhelevskaya, C Ian Mockridge, Christine A Penfold, Patrick J Duriez, Mark S Cragg and 1 more

Abstract read
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In one paragraph

Article in Nature communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Rosendo C Villafuerte-VegaDepartment of Chemistry, University of Michigan, Ann Arbor, MI, USA.
Michael R ArmbrusterDepartment of Chemistry, University of Michigan, Ann Arbor, MI, USA.
Hayden FisherCentre for Cancer Immunology, University of Southampton, Southampton, UK.
Isabel G ElliottCentre for Cancer Immunology, University of Southampton, Southampton, UK.ORCID http://orcid.org/0000-0003-4535-5932
H T Claude ChanCentre for Cancer Immunology, University of Southampton, Southampton, UK.
Tatyana InzhelevskayaCentre for Cancer Immunology, University of Southampton, Southampton, UK.
C Ian MockridgeCentre for Cancer Immunology, University of Southampton, Southampton, UK.
Christine A PenfoldCentre for Cancer Immunology, University of Southampton, Southampton, UK.
Patrick J DuriezCentre for Cancer Immunology, University of Southampton, Southampton, UK.ORCID http://orcid.org/0000-0003-1814-2552
Mark S CraggCentre for Cancer Immunology, University of Southampton, Southampton, UK.ORCID http://orcid.org/0000-0003-2077-089X
Brandon T RuotoloDepartment of Chemistry, University of Michigan, Ann Arbor, MI, USA. bruotolo@umich.edu.ORCID http://orcid.org/0000-0002-6084-2328

Funding

Native Mass Spectrometry Guided Structural Biology CenterRM1GM149374 · NIGMS · OHIO STATE UNIVERSITY · PI Vicki H. Wysocki · 2023 to 2026
$5.0M
NIGMS NIH HHS RM1 GM149374U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences (NIGMS) RM1GM149374
6 · The paper itself

Abstract

Human (h)IgG2 monoclonal antibodies (mAbs) are potent agonists due, in part, to their ability to undergo disulfide shuffling within their hinge regions. Herein, we describe a rapid, sensitive, collision-induced unfolding (CIU) assay that possesses a predictive relationship between gas-phase protein unfolding and agonism in hIgG2 variants. Furthermore, our results highlight the significance of hinge engineering in tuning mAb structure-function relationships for the development of future biotherapeutics.

Indexed as

Antibodies, MonoclonalImmunoglobulin GProtein EngineeringProtein UnfoldingDisulfidesGasesHumansStructure-Activity RelationshipAntibodies, MonoclonalDisulfidesGasesImmunoglobulin G

Identifiers

PMID42373639
PMCPMC13458314

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.